Assessment of Immunological Response and Impacts on Fertility Following Intrauterine Vaccination Delivered to Swine in an Artificial Insemination Dose.

Frontiers in Immunology
Glenn HamonicHeather L Wilson

Abstract

To protect the health of sows and gilts, significant investments are directed toward the development of vaccines against infectious agents that impact reproduction. We developed an intrauterine vaccine that can be delivered with semen during artificial insemination to induce mucosal immunity in the reproductive tract. An in vitro culture of uterine epithelial cells was used to select an adjuvant combination capable of recruiting antigen-presenting cells into the uterus. Adjuvant polyinosinic:polycytidylic acid (poly I:C), alone or in combination, induced expression of interferon gamma, tumor necrosis factor alpha, and select chemokines. A combination adjuvant consisting of poly I:C, host defense peptide and polyphosphazene (Triple Adjuvant; TriAdj), which previously was shown to induce robust mucosal and systemic humoral immunity when administered to the uterus in rabbits, was combined with boar semen to evaluate changes in localized gene expression and cellular recruitment, in vivo. Sows bred with semen plus TriAdj had decreased γδ T cells and monocytes in blood, however, no corresponding increase in the number of monocytes and macrophages was detected in the endometrium. Compared to sows bred with semen alone, sows bred with ...Continue Reading

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Citations

Dec 8, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Alexander K Andrianov, Robert Langer
Mar 26, 2021·Frontiers in Bioengineering and Biotechnology·Dylan J ChandGeorge K Mutwiri
May 11, 2021·Veterinary Immunology and Immunopathology·Kezia R FourieHeather L Wilson

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Methods Mentioned

BETA
lavage
laser-capture microdissection
flow cytometry
UECs
ELISA
motility measurements

Software Mentioned

InterVac
ImageJ
SCA CASA
FlowJo
VIDO
TriAdj CASA
GraphPad
Image J
GraphPad Prism

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