Assessment of in vitro effects of metyrapone on Leydig cell steroidogenesis

Steroids
C Parthasarathy, Karundevi Balasubramanian

Abstract

Metyrapone, a specific inhibitor of 11beta-hydroxylase inhibits glucocorticoid production and it is used in the diagnosis/treatment of hypercortisolism and also to test the functional integrity of hypothalamo-pituitary-adrenal axis. To assess the impact of glucocorticoid deficiency, this drug is preferred over adrenalectomy, which eliminates all the hormonal secretions of the adrenal cortex and medulla. However, whether metyrapone has any direct effect on the extra-adrenocortical cellular or tissue functions remains to be resolved. Our previous study showed a depressed testicular Leydig cell testosterone production in rats treated with metyrapone. Therefore, the present study was designed to examine the possible direct effect of metyrapone on testicular Leydig cell steroidogenesis in vitro. Leydig cell viability and the reactive oxygen species (ROS) concentration were not altered by any of the concentration of metyrapone tested. The efficacy of Leydig cell testosterone production under basal as well as LH-stimulated condition was not altered by metyrapone treatment. Further, Leydig cellular (14)C-glucose oxidation, the activity and mRNA levels of cytochrome side chain cleavage (P(450)scc), 3beta- and 17beta-hydroxysteroid dehyd...Continue Reading

References

Dec 1, 1976·European Journal of Pharmacology·M J Parnham
May 1, 1989·Journal of Steroid Biochemistry·C M FeekC R Edwards
Jan 1, 1988·Annual Review of Physiology·M L Dufau
Aug 1, 1985·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·P P RiekerM B Garnick
Aug 1, 1971·Comparative Biochemistry and Physiology. A, Comparative Physiology·A D Johnson, P C Turner
Jul 15, 1972·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·L A Kraft, A D Johnson
Oct 1, 1982·The Journal of Endocrinology·G LescoatD H Garnier
Jun 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·L BentonM P Hardy
Jul 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·B M Agular, C Vind
Jan 1, 1995·Human & Experimental Toxicology·A J Paine
Mar 1, 1996·Journal of Animal Science·J L Salak-JohnsonR L Norman
Jun 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·D MarićR Kovacević
Jun 1, 1997·The Journal of Steroid Biochemistry and Molecular Biology·R Sampath-KumarK Yang
Jan 31, 1998·Analytical Biochemistry·T Shivanandappa, S Venkatesh
Aug 1, 1998·Biology of Reproduction·S M Mendis-HandagamaR L Haupt
Jun 1, 1999·Nihon Hinyōkika Gakkai zasshi. The japanese journal of urology·M Akiyama
Dec 22, 1999·The Journal of Clinical Endocrinology and Metabolism·S B SeminaraW F Crowley
Feb 26, 2000·Metabolism: Clinical and Experimental·H VierhapperW Waldhäusl
Apr 25, 2000·The Journal of Steroid Biochemistry and Molecular Biology·B R SankarK Balasubramanian
May 29, 2000·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·B R SankarK Balasubramanian
Jun 23, 2001·Molecular and Cellular Endocrinology·R HabertJ M Saez
Sep 18, 2001·The Journal of Biological Chemistry·N S ChandelR H Arch
Oct 4, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·Anthony E MichaelMichael T Rae
Oct 23, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Shirley TengMicheline Piquette-Miller
May 26, 2004·Physiology & Behavior·Jill E Schneider
Dec 15, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·R BadrinarayananK Balasubramanian

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Citations

Mar 10, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Gunda ViswanathPartha Roy
Jul 9, 2009·Molecular and Cellular Endocrinology·Chandrakesan ParthasarathyKarundevi Balasubramanian
Mar 13, 2008·Journal of Cellular Biochemistry·C Parthasarathy, K Balasubramanian
Jan 7, 2015·Environmental Science and Pollution Research International·T A DantasG D C Severi-Aguiar
Mar 4, 2008·Molecular and Cellular Endocrinology·Chandrakesan Parthasarathy, Karundevi Balasubramanian
Apr 25, 2009·Current Opinion in Endocrinology, Diabetes, and Obesity

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