Assessment of in vitro sperm characteristics after flow cytometric sorting of frozen-thawed bull spermatozoa

Theriogenology
F K HollinsheadG Evans

Abstract

The effect of processing prior to sex-sorting, re-freezing and thawing of frozen-thawed bull spermatozoa on in vitro sperm characteristics was investigated. Frozen-thawed bull spermatozoa (three bulls; three ejaculates per bull) were prepared for sorting by washing (FT-WASH) or gradient centrifugation (FT-GRADIENT) and evaluated for motility and forward progressive motility (FPM) after processing, staining, sorting and incubation (3 h; 37 degrees C). After frozen-thawed samples were processed and analyzed using a high-speed cell sorter, aliquots were removed and re-frozen and thawed (FTF-WASH; FTF-GRADIENT). Non-sorted frozen-thawed spermatozoa (FT-CONTROL) were also re-frozen and thawed (FTF-CONTROL). Spermatozoa from all treatments were assessed for penetration of an artificial cervical mucus at 0 h after sorting or thawing, and for motility, FPM and acrosomal status after 3-h incubation (37 degrees C). Frozen-thawed spermatozoa prepared by gradient centrifugation before sorting were sorted more efficiently than washed samples (P < 0.05). However, after sorting (FT) or thawing (FTF) and incubation, the percentage of motile spermatozoa and FPM rating was lower for GRADIENT than WASH (21.5 +/- 3.39%; 1.4 +/- 0.16 FPM versus 48....Continue Reading

Citations

Aug 31, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Roberto PuglisiRiccardo Aleandri
Sep 4, 2010·Veterinary Medicine International·J M Morrell, H Rodriguez-Martinez
Dec 7, 2006·Reproduction, Fertility, and Development·S P de GraafJ K O'Brien
Aug 30, 2011·Reproduction, Fertility, and Development·R KruseJ M Morrell
Jul 3, 2013·Fertility and Sterility·Sofia C RibeiroChristian de Geyter
Dec 6, 2008·Reproduction in Domestic Animals = Zuchthygiene·S L UnderwoodG Evans
Jul 24, 2004·Animal Reproduction Science·W M C MaxwellJ K O'Brien
Oct 26, 2005·Theriogenology·Duane L Garner
Mar 23, 2017·Reproduction in Domestic Animals = Zuchthygiene·T NongbuaJ M Morrell

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