Abstract
This study examined acute toxicity and lung injury following inhalation exposure to ammonia. Male Sprague-Dawley rats (300-350 g) were exposed to 9000, 20,000, 23,000, 26,000, 30,000 or 35,000 ppm of ammonia for 20 min in a custom head-out exposure system. The exposure atmosphere, which attained steady state within 3 min for all ammonia concentrations, was monitored and verified using a Fourier transform infrared spectroscopy (FTIR) gas analyzer. Animals exposed to ammonia resulted in dose-dependent increases in observed signs of intoxication, including increased chewing and licking, ocular irritation, salivation, lacrimation, oronasal secretion and labored breathing. The LCt50 of ammonia within this head-out inhalation exposure model was determined by probit analysis to be 23,672 ppm (16,489 mg/m(3)) for the 20 min exposure in male rats. Exposure to 20,000 or 23,000 ppm of ammonia resulted in significant body weight loss 24-h post-exposure. Lung edema increased in all ammonia-exposed animal groups and was significant following exposure to 9000 ppm. Bronchoalveolar fluid (BALF) protein concentrations significantly increased following exposure to 20,000 or 23,000 ppm of ammonia in comparison to controls. BAL cell (BALC) death an...Continue Reading
References
Jun 30, 1977·International Archives of Occupational and Environmental Health·M M Verberk
Aug 1, 1976·Environmental Health Perspectives·R F Phalen
Jan 1, 1991·Toxicologic Pathology·B T Mossman Vacek
May 1, 1990·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·A AnzuetoS G Jenkinson
May 1, 1970·Toxicology and Applied Pharmacology·R A CoonJ Siegel
Jun 1, 1984·Environmental Health Perspectives·R F PhalenR T Drew
Sep 1, 1982·American Industrial Hygiene Association Journal·L M AppelmanP G Reuzel
Mar 1, 1980·Archives of Otolaryngology·L G CloseA M Cohn
Apr 1, 1980·Chest·T J Montague, A R Macneil
Jun 18, 1998·Archives of Toxicology·A M Sciuto
Mar 17, 1999·Journal of Toxicology. Clinical Toxicology·E SjöblomU Ludwigs
Dec 22, 1999·The American Journal of Gastroenterology·H O Conn
Apr 7, 2000·Burns : Journal of the International Society for Burn Injuries·A GeorgeJ R Kanjoor
May 23, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Isabelle Noël-GeorisRuddy Wattiez
May 20, 2003·Inhalation Toxicology·Alfred M SciutoTed S Moran
Jun 26, 2003·Metabolic Brain Disease·K V Rama RaoD M Norenberg
Oct 1, 1952·Proceedings of the Society for Experimental Biology and Medicine·J H WEATHERBY
Dec 19, 2003·The European Respiratory Journal·P M CalverleyH Olsson
Jan 20, 2004·Proteomics·Isabelle Noël-GeorisRuddy Wattiez
Sep 17, 2004·Archives of Environmental Health·Nachman BrautbarElihu D Richter
Sep 21, 2004·Journal of Bioenergetics and Biomembranes·M D NorenbergA R Jayakumar
Feb 28, 2007·Toxicologic Pathology·Brian A Wong
Jul 23, 2008·Inhalation Toxicology·Xiaohua TianGary W Hoyle
Mar 9, 2010·Toxicology and Applied Pharmacology·Michael W PerkinsMadhusoodana P Nambiar
Mar 5, 2011·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Dylan W de Lange, Jan Meulenbelt
May 3, 2011·American Journal of Respiratory Cell and Molecular Biology·Gustavo Matute-BelloUNKNOWN Acute Lung Injury in Animals Study Group
Dec 27, 2011·Neurological Research·Adam BackJames R Brorson
Nov 13, 2012·Neurochemistry International·Marta Skowrońska, Jan Albrecht
Nov 13, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Wenli LiJuergen Pauluhn
Dec 26, 2012·Toxicology·Lingyi ZhangGaku Ichihara
Jan 31, 2013·Scientific Reports·Rong LanShanwen Tao
Apr 16, 2013·Toxicology Mechanisms and Methods·Benjamin WongAlfred M Sciuto
May 28, 2013·Regulatory Toxicology and Pharmacology : RTP·Jürgen Pauluhn
Jul 28, 2013·Chemico-biological Interactions·Michael W PerkinsAlfred M Sciuto
May 3, 2014·Journal of Burn Care & Research : Official Publication of the American Burn Association·Shiara Ortiz-PujolsBruce A Cairns
Citations
May 2, 2019·Inhalation Toxicology·Linda ElfsmarkSofia Jonasson
Nov 26, 2019·Journal of Occupational Health·Zhizhen XuZhaohui Fu
Feb 12, 2017·Inhalation Toxicology·Michael W PerkinsAlfred M Sciuto
Oct 30, 2018·Dose-response : a Publication of International Hormesis Society·Abdul Rohim TualekaRizky Maharja
Jun 13, 2021·Toxicology Letters·Lina ÅgrenSofia Jonasson
Nov 23, 2021·Environmental Toxicology·Wenhao QinZuojun Tan