Abstract
Timely diagnosis of ischaemia-reperfusion (IR)-induced injury after testicular torsion may be critical for saving reproductive function. The purpose of this study was to detect IR-induced injury, indicated by E-selectin overexpression, in murine testis using ultrasound molecular contrast imaging. Mice underwent 720(°) unilateral testicular torsion (ischaemia) followed by detorsion (reperfusion), and the control group (Sham-IR) was operated identically without extended ischaemia. In a separate positive control group, TNF-α was injected intratesticularly to induce inflammation and compared to intratesticular saline injection. Selectin-targeted or nontargeted ultrasound contrast microbubbles were injected intravenously, and two-dimensional (2D) real-time high-resolution ultrasound testicular imaging was performed after reperfusion or after TNF-α injection. Contrast intensity levels were significantly higher in the testis of the IR group as compared to the Sham-IR group after injection of targeted contrast microbubbles. Contrast intensities were similar between the IR and Sham-IR groups after injection of nontargeted microbubbles. In addition, targeted contrast intensity levels were significantly higher in the TNF-α-treated group a...Continue Reading
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