PMID: 8943095Dec 1, 1996Paper

Assessment of laminin-mediated glioma invasion in vitro and by glioma tumors engrafted within rat spinal cord

Journal of Neuro-oncology
D MuirBernard L Maria

Abstract

Cell motility within central nervous system (CNS) neuropil may be largely restricted yet infiltration by glioma cells is commonly observed. Glioma cells remodel nervous tissue and may assemble extracellular matrix in order to migrate. We examined the rat C6 glioma cell line for laminin expression and response in vitro and following engraftment into rat spinal cord. C6 cell cultures expressed laminin-2. C6 cells attached equally well to substrates of purified laminin-1 and laminin-2 and laminin-2-enriched C6 conditioned medium. In contrast, C6 cell migration was substantially greater on laminin-2 and C6-derived substrata than on laminin-1. Glioma cell attachment to laminin-1 and -2 was largely inhibited by antibody to the laminin receptor LBP110 and by an IKVAV peptide but not by YIGSR or control peptides. IKVAV peptide and anti-LBP110 antibodies also inhibited glioma cell invasion through synthetic basement membrane. Anti-beta 1 integrin antibody selectively inhibited cell migration and invasion on laminin-2 substrata without affecting percent cell attachment. These findings suggest C6 cell migration and invasion are promoted by autocrine release of laminin-2 and involve LPB110 and beta 1 integrin laminin receptors. A possible ...Continue Reading

Citations

Aug 23, 2005·BMC Cell Biology·Claudia Beatriz Nedel Mendes de AguiarAndréa Gonçalves Trentin
Aug 10, 2000·Neoplasia : an International Journal for Oncology Research·M E Berens, A Giese
Oct 9, 2002·Oncogene·Hassan M Fathallah-ShaykhElia DiSavino
Jul 11, 2006·Expert Review of Molecular Diagnostics·Bodour SalhiaMichael E Berens
Jun 27, 2000·Journal of Cellular Physiology·C K RitchieK Khalili
Jan 1, 1998·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·R Douglas Fields
Feb 3, 1998·Seminars in Pediatric Neurology·D F Muir

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