Assessment of migration of HIV-1-loaded dendritic cells labeled with 111In-oxine used as a therapeutic vaccine in HIV-1-infected patients

Immunotherapy
Alba RuizF García

Abstract

Monocyte-derived dendritic cells (DCs) loaded with heat-inactivated HIV are used in therapeutic immunizations. It is not known whether they migrate in vivo to lymph nodes. We used an (111)In-oxine-labeled DC (ILDC) method to visualize the migration of DCs. The activity, time and incubation medium were investigated to obtain the highest cellular viability and radiolabeling yield. A trypan-blue exclusion test was used to determine the cellular viability. In five patients, 2 x 10(6) ILDCs were injected subcutaneously in the arm. An initial dynamic study was performed during the first 5 min after injection. This was followed by static acquisitions at several time points, using a high-resolution (general electric) gamma-camera and quantifying the activity at regions of interest drawn on the injection point. The sensitivity of the gamma-camera was evaluated. The highest number of viable DCs (>83%) and the best radiolabeling yield (>70%) were obtained with 1.11 MBq (111)In-oxine, after 10 min of incubation at 37 degrees C in sodium chloride solution 0.9%. We did not observe migration of ILDCs to local lymph nodes in any patient. However, focal uptake at the place of injection continued during the study period. We observed a higher tha...Continue Reading

References

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Citations

Dec 21, 2010·European Journal of Nuclear Medicine and Molecular Imaging·Luisa OttobriniGiovanni Lucignani
Jan 4, 2013·Science Translational Medicine·Felipe GarcíaUNKNOWN DCV2/MANON07-ORVACS Study Group
Jul 20, 2010·Immunotherapy·Telma Miyuki OshiroAlberto José da Silva Duarte
Apr 23, 2017·Expert Review of Vaccines·Lorna LealFelipe García
May 26, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Peter GawneRafael T M de Rosales

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
biopsies
biopsy

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