Assessment of Migration of Human MSCs through Fibrin Hydrogels as a Tool for Formulation Optimisation.

Materials
Nasseem SalamSimon Partridge

Abstract

Control of cell migration is fundamental to the performance of materials for cell delivery, as for cells to provide any therapeutic effect, they must migrate out from the delivery material. Here the influence of fibrinogen concentration on the migration of encapsulated human mesenchymal stem cells (hMSCs) from a cell spheroid through fibrin hydrogels is tracked over time. Fibrin was chosen as a model material as it is routinely employed as a haemostatic agent and more recently has been applied as a localised delivery vehicle for potential therapeutic cell populations. The hydrogels consisted of 5 U/mL thrombin and between 5 and 50 mg/mL fibrinogen. Microstructural and viscoelastic properties of different compositions were evaluated using SEM and rheometry. Increasing the fibrinogen concentration resulted in a visibly denser matrix with smaller pores and higher stiffness. hMSCs dispersed within the fibrin gels maintained cell viability post-encapsulation, however, the migration of cells from an encapsulated spheroid revealed that denser fibrin matrices inhibit cell migration. This study provides the first quantitative study on the influence of fibrinogen concentration on 3D hMSC migration within fibrin gels, which can be used to...Continue Reading

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Citations

Aug 16, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yiyuan DuanChangyou Gao
Jun 17, 2020·Macromolecular Bioscience·Antonina LavrentievaIliyana Pepelanova
May 9, 2021·Colloids and Surfaces. B, Biointerfaces·Colleen A RoosaAshley C Brown
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Dec 4, 2021·Journal of Tissue Engineering and Regenerative Medicine·Katja HölzlSylvia Nürnberger

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