Assessment of morphological and immunohistological alterations in long-term keloid skin explants

Cells, Tissues, Organs
Hai S DuongDiana V Messadi

Abstract

One of the major impediments in keloid research is the lack of a keloid animal model that can mimic human keloid. This imposes investigative constraints on studying cellular interactions and biochemical processes that normally occur in vivo. Our main objective is to establish an in vitro model for maintaining long-term viable keloid dermal explants as a tool for investigating the pathogenesis of keloid scar formation. Explants of adult keloid scars were cultured in vitro by embedding them in enriched collagen gel matrix and maintaining them for up to 6 weeks, whereupon changes in tissue morphology and cellular differentiation were examined. The effects of medium enrichment, air versus liquid submersion, and different substrates on the explants were examined. Our results indicated that keloid explants embedded in a collagen gel matrix were morphologically better preserved than explants placed on a plastic substrate. Explants with epidermis at the air-liquid interface had better morphology than collagen-submerged explants, and there were no differences between serum-free and serum-supplemented explant cultures. Immunohistochemical and apoptotic analyses were performed to assess cellular viability and differentiation. In situ hybr...Continue Reading

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Citations

Apr 19, 2012·Experimental Dermatology·Rania BagabirArdeshir Bayat
Apr 18, 2016·Archives of Dermatological Research·G P SidgwickA Bayat
Jun 25, 2016·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Wenbo WangYue-Lei Chen
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Dec 12, 2012·Journal of Burn Care & Research : Official Publication of the American Burn Association·Haibin Wang, Shengkang Luo
Mar 2, 2018·Tissue Engineering. Part C, Methods·Grace C LimandjajaSusan Gibbs
Jul 10, 2013·Laboratory Investigation; a Journal of Technical Methods and Pathology·Farhatullah SyedArdeshir Bayat
Jun 13, 2020·Frontiers in Cell and Developmental Biology·Grace C LimandjajaSusan Gibbs
Sep 12, 2019·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Jialun LiJiaming Sun

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