Assessment of Multi Fragment Melting Analysis System (MFMAS) for the Identification of Food-Borne Yeasts

Current Microbiology
Z KesmenHasan Yetim

Abstract

Multi Fragment Melting Analysis System (MFMAS) is a novel approach that was developed for the species-level identification of microorganisms. It is a software-assisted system that performs concurrent melting analysis of 8 different DNA fragments to obtain a fingerprint of each strain analyzed. The identification is performed according to the comparison of these fingerprints with the fingerprints of known yeast species recorded in a database to obtain the best possible match. In this study, applicability of the yeast version of the MFMAS (MFMAS-yeast) was evaluated for the identification of food-associated yeast species. For this purpose, in this study, a total of 145 yeast strains originated from foods and beverages and 19 standard yeast strains were tested. The DNAs isolated from these yeast strains were analyzed by the MFMAS, and their species were successfully identified with a similarity rate of 95% or higher. It was shown that the strains belonged to 43 different yeast species that are widely found in the foods. A clear discrimination was also observed in the phylogenetically related species. In conclusion, it might be suggested that the MFMAS-yeast seems to be a highly promising approach for a rapid, accurate, and one-ste...Continue Reading

References

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Mar 5, 2016·Journal of Clinical Microbiology·Hana ObručováTomáš Freiberger
Aug 31, 2016·The Journal of Microbiology·Mine ErdemHasan Yetim

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Citations

May 25, 2021·Computer Methods and Programs in Biomedicine·Fatma Ozge Ozkok, Mete Celik

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Methods Mentioned

BETA
PCR

Software Mentioned

Multi Fragment Melting Analysis System ( MFMAS )
MFMAS
yeast
SQL Database

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