Assessment of novel tobacco heating product THP1.0. Part 6: A comparative in vitro study using contemporary screening approaches

Regulatory Toxicology and Pharmacology : RTP
Mark TaylorMarianna D Gaça

Abstract

Cigarette smoking is a major risk factor for many adverse health conditions. Novel tobacco heating products (THPs) heat tobacco, reducing exposure to many of the harmful combustion toxicants in conventional cigarette emissions. In vitro studies have been employed to support the toxicological evaluation of chemicals and complex mixtures, including cigarette smoke. The use of automated robotics platforms for in vitro toxicological screening complements traditional testing approaches. Multiparametric toxicity and oxidative stress endpoints were used to assess in vitro biological responses elicited after exposure to total particulate matter (TPM) from two commercially available THPs, and the reference tobacco product 3R4F, in human bronchial epithelial cells. A luciferase-based reporter gene assay was used to assess antioxidant response element (ARE) transcriptional activation in stably transfected H292 cells after 6 and 24 h exposures. High-content screening was used to assess 10 endpoints normal human bronchial epithelial cells after 4 or 24 h exposures. 3R4F TPM stimulated significant increases in ARE activation (p < 0.005) and moderate activity in HCS cell-based assays compared to THP at comparable doses. THPs showed little or ...Continue Reading

Citations

Oct 28, 2019·Frontiers in Public Health·Nadja MallockAndreas Luch
Feb 16, 2019·Internal and Emergency Medicine·Julia HoengManuel C Peitsch
Oct 1, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Bishop EGaca M
Sep 29, 2020·Toxicology Reports·D BrehenyM Gaça
Mar 14, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Mariana Reis ArantesJose Tadeu Abreu Oliveira
Apr 14, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Hongjuan WangQingyuan Hu
Nov 16, 2018·Revue des maladies respiratoires·B Dautzenberg, M-D Dautzenberg

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