PMID: 11925398Apr 2, 2002Paper

Assessment of nuclear and cytoplasmic maturation in in-vitro matured human oocytes

Human Reproduction
Catherine M H CombellesDavid F Albertini

Abstract

With improved prospects for the use of human oocyte in-vitro maturation in assisted reproductive technologies, the need to define more clearly the coordination of nuclear and cytoplasmic maturation has arisen. Immunofluorescence and confocal microscopy were used to evaluate cell cycle-dependent modifications in chromatin and microtubules in human germinal vesicle oocytes (n = 455) undergoing in-vitro maturation. Four distinct classes of germinal vesicle stage oocytes were identified based on the expression of G2/interphase characteristics, but, of these, only one class of oocytes was competent to complete meiotic progression to metaphase-II in vitro. The majority of germinal vesicle stage oocytes resumed meiosis within 6 h (88.9%) of culture and exhibited an accelerated pace of progression to metaphase-II (66.7%) over 24 h, but in general were unable to maintain meiotic arrest and defaulted into interphase within 24 h of polar body emission. Characterization of microtubule dynamics and chromatin phosphorylation demonstrates specific cell cycle deficiencies in in-vitro matured human oocytes. This work forms a basis for future studies aimed at optimizing nuclear and cytoplasmic maturation during in-vitro maturation.

Citations

Oct 23, 2010·Journal of Assisted Reproduction and Genetics·Laura EscrichMaría-José Escribá
Nov 18, 2011·Journal of Assisted Reproduction and Genetics·Catherine M H CombellesCatherine Racowsky
Feb 23, 2012·Journal of Assisted Reproduction and Genetics·Aijun ZhangChen Xu
Jul 17, 2012·Journal of Assisted Reproduction and Genetics·Sulochana GunasheelaWeon-Young Son
Jul 31, 2013·Journal of Assisted Reproduction and Genetics·Yukitaka MurakoshiYasunori Yoshimura
Aug 11, 2004·Fertility and Sterility·Helena FulkaJosef Fulka
Jul 2, 2003·Reproductive Biomedicine Online·David F AlbertiniCatherine M H Combelles
Feb 18, 2011·Zygote : the Biology of Gametes and Early Embryos·Bita EbrahimiHossein Baharvand
May 26, 2009·Human Reproduction·J J BromfieldD F Albertini
Jul 15, 2009·Human Reproduction·Min XuTeresa K Woodruff
May 18, 2010·Human Reproduction·Catherine M H CombellesCatherine Racowsky
Jan 13, 2011·Human Reproduction·Catherine M H CombellesCatherine Racowsky
Sep 13, 2012·Human Reproduction·Ronit MachtingerCatherine Racowsky
Aug 2, 2013·Human Reproduction·Ronit MachtingerCatherine Racowsky
Oct 17, 2013·Human Reproduction·Giovanni CoticchioDavid F Albertini
Nov 21, 2008·Molecular Human Reproduction·Jing-He TanJie Zhang
Sep 5, 2003·Biology of Reproduction·Sandra CecconiBarbara Barboni
Jan 11, 2008·Biology of Reproduction·Andrea DiLuigiLisa M Mehlmann
Jun 17, 2003·Clinical Obstetrics and Gynecology·Douglas T CarrellC Matthew Peterson
Oct 6, 2010·Reproductive Biology and Endocrinology : RB&E·Olga Karapanou, Anastasios Papadimitriou
Jan 15, 2011·Journal of Human Reproductive Sciences·Zhi Qin ChenHans Ingolf Nielsen
Feb 23, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Imene BoumelaSamir Hamamah
Nov 22, 2013·Molecular Human Reproduction·Giovanni CoticchioRubens Fadini
Oct 5, 2010·Veterinary Medicine International·Jennifer R Prentice, Muhammad Anzar
Oct 9, 2014·Journal of Assisted Reproduction and Genetics·Yu-Hung LinChii-Ruey Tzeng
Nov 26, 2014·Reproductive Biology and Endocrinology : RB&E·Maria G PalmeriniGuido Macchiarelli
Aug 21, 2012·Fertility and Sterility·Laura EscrichMaría-José Escribá

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