Assessment of okadaic acid effects on cytotoxicity, DNA damage and DNA repair in human cells

Mutation Research
Vanessa ValdiglesiasBlanca Laffon

Abstract

Okadaic acid (OA) is a phycotoxin produced by several types of dinoflagellates causing diarrheic shellfish poisoning (DSP) in humans. Symptoms induced by DSP toxins are mainly gastrointestinal, but the intoxication does not appear to be fatal. Despite this, this toxin presents a potential threat to human health even at concentrations too low to induce acute toxicity, since previous animal studies have shown that OA has very potent tumour promoting activity. However, its concrete action mechanism has not been described yet and the results reported with regard to OA cytotoxicity and genotoxicity are often contradictory. In the present study, the genotoxic and cytotoxic effects of OA on three different types of human cells (peripheral blood leukocytes, HepG2 hepatoma cells, and SHSY5Y neuroblastoma cells) were evaluated. Cells were treated with a range of OA concentrations in the presence and absence of S9 fraction, and MTT test and Comet assay were performed in order to evaluate cytotoxicity and genotoxicity, respectively. The possible effects of OA on DNA repair were also studied by means of the DNA repair competence assay, using bleomycin as DNA damage inductor. Treatment with OA in absence of S9 fraction induced not statistica...Continue Reading

References

Jun 1, 1991·Environmental Health Perspectives·H FujikiT Sugimura
Mar 1, 1991·Mutation Research·L F Povirk, M J Austin
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M SuganumaT Sugimura
Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Nov 14, 1997·Bioorganic & Medicinal Chemistry·J E SheppeckA R Chamberlin
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·H NakagamaM Nagao
Sep 9, 2000·Toxicon : Official Journal of the International Society on Toxinology·G BervenP O Seglen
May 29, 2001·The Journal of Biological Chemistry·P DouglasS P Lees-Miller
Nov 2, 2001·Toxicon : Official Journal of the International Society on Toxinology·Emiko ItoKenichi Harada
Oct 22, 2002·The Journal of Laboratory and Clinical Medicine·Michal HermanUzi Gafter
Nov 14, 2002·Biochemical Pharmacology·Paola PoliTânia Maria Araújo Domingues Zucchi
Jun 14, 2003·Toxicological Sciences : an Official Journal of the Society of Toxicology·Susan L StarcevicRaymond F Novak
Nov 6, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·D CavalloS Iavicoli
Dec 19, 2003·Mutagenesis·Giselle Pérez MachadoRicard Marcos
Dec 24, 2003·Teratogenesis, Carcinogenesis, and Mutagenesis·Eduardo CemeliRicardo Marcos
Apr 6, 2004·Current Opinion in Chemical Biology·Jingyang Chen, JoAnne Stubbe
Feb 8, 2006·Environmental Toxicology·Ludovic Le HégaratValérie Fessard
Jul 11, 2006·International Journal of Environmental Research and Public Health·Pinto-Silva CarvalhoEdmond E Creppy
Jul 3, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sushila PatelAlok Dhawan
Sep 18, 2007·Organic Letters·Antonio Hernández DaranasJosé J Fernández
Oct 16, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Lin AoJia Cao
Jun 10, 2008·Toxicon : Official Journal of the International Society on Toxinology·Ghada Souid-MensiEdmond E Creppy
Mar 28, 2009·International Journal of Molecular Sciences·Pholo W MaenetjeMike F Dutton
May 27, 2009·Biological Trace Element Research·Blanca LaffonJosefina Méndez
Sep 8, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Inge NelissenGreet Schoeters
Apr 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C G RogersI Langlois

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Citations

Jun 29, 2011·Journal of Toxicology and Environmental Health. Part a·Fernanda Flórez-BarrósJuan Fernández-Tajes
Jun 29, 2011·Journal of Toxicology and Environmental Health. Part a·Vanessa ValdiglesiasJosefina Méndez
Nov 5, 2013·Marine Drugs·Vanessa ValdiglesiasBlanca Laffon
Nov 15, 2013·Toxins·Rex Munday, John Reeve
Jul 14, 2012·Journal of Toxicology and Environmental Health. Part a·Vanessa ValdiglesiasBlanca Laffon
Dec 3, 2014·Marine Environmental Research·Moira McCarthyFrank F N A M van Pelt
Apr 9, 2013·Ecotoxicology and Environmental Safety·Vanessa ValdiglesiasBlanca Laffon
Sep 25, 2012·Marine Environmental Research·Maria Prado-AlvarezJuan Fernandez-Tajes
Mar 15, 2011·Toxicon : Official Journal of the International Society on Toxinology·Vanessa ValdiglesiasJosefina Méndez
Sep 29, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Rodrigo González-RomeroJosé M Eirín-López
Mar 3, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Yuquan LuTomotaka Sobue
Nov 23, 2012·Toxicological and Environmental Chemistry·Lynne A FieberKathleen S Rein
Apr 29, 2011·Journal of Environmental Monitoring : JEM·Vanessa ValdiglesiasJosefina Méndez
Jul 15, 2015·Journal of Toxicology and Environmental Health. Part a·María Verónica Prego-FaraldoJosefina Méndez
Aug 12, 2018·Toxins·Natalia VilariñoLuis M Botana

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