Assessment of ORAI1-mediated basal calcium influx in mammary epithelial cells

BMC Cell Biology
Diana G F RossGregory R Monteith

Abstract

The entry of calcium ions into mammary gland epithelial cells is one of the least well-understood processes in the transport of calcium into milk during lactation. The store-operated calcium entry channel ORAI1, has been suggested as a potential mechanism for the entry of Ca(2+) into mammary gland epithelial cells from the maternal blood supply during lactation. The down regulation of the canonical ORAI1 activator STIM1 during lactation suggests that other known ORAI activators such as STIM2 and SPCA2 may be important during lactation. Differentiation of HC11 mammary gland epithelial cells was associated with enhanced basal Ca(2+) influx. Silencing of Orai1 abolished this enhancement of Ca(2+) influx. Stim2 had a modest effect on Ca(2+) influx in this in vitro model of lactation, whereas Stim1 and Spca2 silencing had no effect. Despite pronounced increases in Spca2 mRNA during lactation there was no change in the generation of the alternative splice product generated by Mist1, which increases during lactation. These studies support the hypothesis that lactation is associated with a remodelling of Ca(2+) influx and this is associated with enhancement of basal Ca(2+) influx. This enhanced Ca(2+) influx appears to occur through th...Continue Reading

References

Mar 1, 1989·Environmental Health Perspectives·F Borellini, T Oka
Mar 1, 1983·Journal of Dairy Science·M C Neville, C D Watters
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K G DanielsonD Medina
Jun 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C Q Lin, M J Bissell
Apr 13, 1999·The American Journal of Physiology·T A Reinhardt, R L Horst
Jul 14, 2000·Physiological Reviews·D B Shennan, M Peaker
Oct 13, 2000·American Journal of Physiology. Cell Physiology·T A ReinhardtR L Horst
Mar 8, 2003·The Journal of Biological Chemistry·Michael C Chicka, Emanuel E Strehler
Apr 10, 2003·Pflügers Archiv : European journal of physiology·Joost G J HoenderopRené J M Bindels
Apr 23, 2003·Advanced Drug Delivery Reviews·James L McManaman, Margaret C Neville
Apr 23, 2004·Current Molecular Medicine·Emanuel E Strehler, Marek Treiman
Aug 11, 2004·The Journal of Biological Chemistry·Timothy A ReinhardtRonald L Horst
Apr 16, 2005·The Journal of Biological Chemistry·Jo VanoevelenFrank Wuytack
Jul 12, 2005·Current Biology : CB·Jen LiouTobias Meyer
Jul 19, 2005·Journal of Mammary Gland Biology and Neoplasia·Margaret C Neville
Jan 18, 2006·Biochimica Et Biophysica Acta·Won Jae LeeSarah J Roberts-Thomson
Jun 29, 2006·Journal of Cell Science·M'hamed GratiRobert J Wenthold
Oct 19, 2006·Biochemical and Biophysical Research Communications·Pingyong XuTao Xu
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jen LiouTobias Meyer
Nov 14, 2007·Journal of Mammary Gland Biology and Neoplasia·Joshua N VanHouten, John J Wysolmerski
Mar 13, 2008·Biochemical and Biophysical Research Communications·Helen M FaddyGregory R Monteith
Jan 13, 2011·Molecular Cancer Therapeutics·Damara McAndrewGregory R Monteith

❮ Previous
Next ❯

Citations

Apr 23, 2015·Proceedings of the National Academy of Sciences of the United States of America·Felicity M DavisJames W Putney
Oct 18, 2016·Frontiers in Physiology·Wonnam Kim, John J Wysolmerski
May 19, 2017·European Biophysics Journal : EBJ·Olivier MignenChristophe Vandier
Dec 9, 2017·Advances in Biological Regulation·James W Putney

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
Assay

Software Mentioned

Prism Graph Pad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Current Topics in Membranes
Aparna GudlurPatrick G Hogan
Biochemical Society Transactions
Jeremy T Smyth, James W Putney
Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology
Lin LinWeidong Qi
Molecular Cancer Therapeutics
Damara McAndrewGregory R Monteith
© 2021 Meta ULC. All rights reserved