Assessment of Outer Retinal Remodeling in the Hibernating 13-Lined Ground Squirrel

Investigative Ophthalmology & Visual Science
Benjamin S SajdakJoseph Carroll

Abstract

We examined outer retinal remodeling of the euthermic and torpid cone-dominant 13-lined ground squirrel (13-LGS) retina using optical coherence tomography (OCT) imaging and histology. Retinas and corneas of living 13-LGSs were imaged during euthermic and torpid physiological states using OCT. Retinal layer thickness was measured at the visual streak from registered and averaged vertical B-scans. Following OCT, some retinas were collected immediately for postmortem histologic comparison using light microscopy, immunofluorescence, or transmission electron microscopy. Compared to OCT images from euthermic retinae, OCT images of torpid retinae revealed significantly thicker inner and outer nuclear layers, as well as increases in the distances between outer retinal reflectivity bands 1 and 2, and bands 3 and 4. A significant decrease in the distance between bands 2 and 3 also was seen, alongside significant thinning of the choriocapillaris and choroid. OCT image quality was reduced in torpid eyes, partly due to significant thickening of the corneal stroma during this state. The torpid retina of the hibernating 13-LGS undergoes structural changes that can be detected by OCT imaging. Comparisons between in vivo OCT and ex vivo histomo...Continue Reading

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Citations

Aug 2, 2019·Investigative Ophthalmology & Visual Science·Petr SoukupSimon Pot
Jul 11, 2020·Investigative Ophthalmology & Visual Science·Andreas PollreiszChristine A Curcio
Jun 12, 2020·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Cleildo P SantanaArmando da Silva-Cunha
Dec 20, 2019·Biomedical Optics Express·Alexander Meadway, Lawrence C Sincich
Dec 24, 2019·Translational Vision Science & Technology·Alison L HuckenpahlerMaureen A McCall
Sep 10, 2019·Progress in Retinal and Eye Research·Daniela M VerraDavid Hicks
Mar 4, 2019·Vision Research·Benjamin S SajdakJoseph Carroll
Jul 1, 2020·Seminars in Cell & Developmental Biology·Wei Li
Jul 8, 2021·Translational Vision Science & Technology·Alexander E SalmonJoseph Carroll
Jul 27, 2021·Experimental Biology and Medicine·Mina GaffneyJoseph Carroll

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Methods Mentioned

BETA
light microscopy
transmission electron microscopy
scanning electron microscopy
light scattering

Software Mentioned

InStat
Photoshop
ImageJ
OCT Reflectivity Analytics
GraphPad
Bioptigen InVivoVue

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