Assessment of partial sequencing of the 65-kilodalton heat shock protein gene (hsp65) for routine identification of Mycobacterium species isolated from clinical sources

Journal of Clinical Microbiology
Alan McNabbJudith Isaac-Renton

Abstract

We assessed the ability of an in-house database, consisting of 111 hsp65 sequences from putative and valid Mycobacterium species or described groups, to identify 689 mycobacterial clinical isolates from 35 species or groups. A preliminary assessment indicated that hsp65 sequencing confirmed the identification of 79.4% of the isolates from the 32 species examined, including all Mycobacterium tuberculosis complex isolates, all isolates from 13 other species, and 95.6% of all M. avium-M. intracellulare complex isolates. Identification discrepancies were most frequently encountered with isolates submitted as M. chelonae, M. fortuitum, M. gordonae, M. scrofulaceum, and M. terrae. Reexamination of isolates with discrepant identifications confirmed that hsp65 identifications were correct in a further 40 isolates. This brought the overall agreement between hsp65 sequencing and the other identification methods to 85.2%. The remaining 102 isolates had sequence matches below our acceptance criterion, had nondifferential sequence matches between two or more species, were identified by 16S rRNA sequencing as a putative taxonomic group not contained in our database, or were identified by hsp65 and 16S rRNA gene sequencing as a species not in...Continue Reading

References

Jul 1, 1989·Molecular Microbiology·A J HanceB Gicquel
Apr 1, 1993·International Journal of Systematic Bacteriology·J KazdaZ S Tarnok
Jul 1, 1995·International Journal of Systematic Bacteriology·P Koukila-KähköläM L Katila
Jan 1, 1996·Journal of Clinical Microbiology·H SoiniM K Viljanen
Jan 1, 1997·Journal of Clinical Microbiology·M PicardeauV Vincent
Apr 1, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E TortoliE C Böttger
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Oct 23, 1997·International Journal of Systematic Bacteriology·H ShojaeiC G Brignall
Oct 23, 1997·International Journal of Systematic Bacteriology·D van SoolingenJ D van Embden
Dec 17, 1998·Journal of Clinical Gastroenterology·D L PatersonI R Marino
Feb 13, 1999·Journal of Clinical Microbiology·H RinguetC Pierre-Audigier
Mar 13, 1999·Journal of Clinical Microbiology·G G HoggJ M Brown
Feb 3, 2000·Journal of Clinical Microbiology·M L BeggsK D Eisenach
Apr 4, 2000·Journal of Clinical Microbiology·T StinearP D Johnson
Oct 4, 2000·Journal of Clinical Microbiology·J WolfeA Kabani
Jan 11, 2000·Nucleic Acids Research·D L WheelerB A Rapp
Jan 4, 2001·Journal of Clinical Microbiology·N D Friedman, D J Sexton
Jun 28, 2001·Journal of Clinical Microbiology·S SuomalainenK Mattson
Jun 28, 2001·Journal of Clinical Microbiology·P S Conville, F G Witebsky
Aug 14, 2001·Journal of Clinical Microbiology·E RichterD Harmsen

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Citations

Feb 19, 2013·Epidemiology and Infection·B L OngK Verasahib
Oct 12, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Stephan SchulzFalko Fend
Apr 13, 2007·Clinical Microbiology Reviews·Christine Y TurenneMarcel A Behr
May 1, 2009·Journal of Clinical Microbiology·Rafael Silva DuarteJorge Luiz Mello Sampaio
Sep 7, 2006·Journal of Clinical Microbiology·Jorge Luiz Mello SampaioSylvia Cardoso Leão
Nov 5, 2010·Journal of Clinical Microbiology·Jianli DaiJudith A Johnson
Sep 28, 2007·Journal of Clinical Microbiology·Eun-Young JangJun Hee Woo
Sep 28, 2007·Journal of Clinical Microbiology·Luiz Claudio Oliveira LazzariniJohn L Ho
Apr 24, 2009·Journal of Clinical Microbiology·Enrico TortoliMichela Pascarella
Dec 12, 2012·Journal of Clinical Microbiology·Nandhakumar BalakrishnanEdward B Breitschwerdt
Apr 1, 2011·Journal of Clinical Microbiology·Jianli DaiMichael Lauzardo
Mar 8, 2005·Journal of Clinical Microbiology·Adrian M ZelaznySteven H Fischer
Feb 4, 2006·Journal of Clinical Microbiology·Christine Y TurenneMarcel A Behr
Feb 6, 2007·American Journal of Respiratory and Critical Care Medicine·David E GriffithUNKNOWN Infectious Disease Society of America
May 10, 2013·BMC Veterinary Research·Tyler C ThackerWade Ray Waters
Oct 16, 2010·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Yoon-Seok RohBumseok Kim
Mar 12, 2011·Emerging Infectious Diseases·Eva LudwigWalter Hermanns
Sep 21, 2013·Emerging Infectious Diseases·Abdolrazagh Hashemi-ShahrakiEnrico Tortoli
Aug 25, 2012·Lung India : Official Organ of Indian Chest Society·Vijay Nema
Dec 14, 2011·Revue des maladies respiratoires·C AndréjakV Jounieaux
Aug 12, 2014·Systematic Biology·Frederick A Matsen
Aug 14, 2012·Journal of Comparative Pathology·C PalmieriH L Shivaprasad
Sep 20, 2011·Enfermedades infecciosas y microbiología clínica·Óscar EsparciaPere Coll
Oct 5, 2010·Diagnostic Microbiology and Infectious Disease·Ana Roberta Fusco da CostaKarla Valéria Batista Lima
Oct 3, 2009·Diagnostic Microbiology and Infectious Disease·Ana Roberta Fusco da CostaKarla Valéria Batista Lima
Sep 5, 2006·Journal of Fish Diseases·I M KaattariE B Shotts
Nov 6, 2009·American Journal of Physical Anthropology·Anne C StoneCharlotte A Roberts

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