Assessment of pig saliva as a Streptococcus suis reservoir and potential source of infection on farms by use of a novel quantitative polymerase chain reaction assay

American Journal of Veterinary Research
Sakura AraiTsutomu Sekizaki

Abstract

OBJECTIVE To evaluate colonization of Streptococcus suis and Streptococcus parasuis on pig farms in Japan and to identify sources of infections. SAMPLE Saliva, feces, and vaginal swab samples from 84 healthy pigs of several growth stages on 4 farms and swab samples of feed troughs and water dispensers at those farms. PROCEDURES Samples were collected from August 2015 to June 2016. Two quantitative PCR (qPCR) assays (one for S suis and the other for S parasuis) were designed for use in the study. The novel qPCR assays were used in combination with previously described qPCR assays for S suis serotype 2 or 1/2 and total bacteria. Relative abundance of bacteria in each sample was evaluated. RESULTS Streptococcus suis was detected in all saliva samples and some of the other samples, whereas S parasuis was not detected in any of the samples, including saliva samples, which indicated a difference in colonization preference. The ratio of S suis to total bacteria in saliva appeared to increase with age of pigs. Streptococcus suis serotype 2 or 1/2 was detected in a few saliva samples and feed trough swab samples at 2 farms where S suis infections were prevalent. CONCLUSIONS AND CLINICAL RELEVANCE Saliva, especially that of sows, appeare...Continue Reading

References

Jul 1, 1990·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·R Higgins, M Gottschalk
Jun 1, 1997·Journal of Clinical Microbiology·S F AmassL K Clark
Nov 6, 2004·Journal of Clinical Microbiology·C KasorndorkbuaP G Halbur
Apr 7, 2006·Applied and Environmental Microbiology·Kirsti M RitalahtiFrank E Löffler
May 19, 2006·Emerging Infectious Diseases·Hongjie YuUNKNOWN Streptococcus suis study groups
Feb 5, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Heiman F L WertheimConstance Schultsz
Jul 20, 2011·Diagnostic Microbiology and Infectious Disease·Tran Vu Thieu NgaConstance Schultsz
Jun 12, 2012·Applied and Environmental Microbiology·Marta Gómez-DoñateAnicet R Blanch
Aug 10, 2012·Journal of Clinical Microbiology·Kaicheng WangChengping Lu
Mar 19, 2014·Applied and Environmental Microbiology·Laetitia BonifaitCaroline Duchaine
May 21, 2014·Proceedings of the National Academy of Sciences of the United States of America·Eric A FranzosaCurtis Huttenhower
Sep 18, 2014·Journal of Microbiological Methods·Sakura IshidaTsutomu Sekizaki
Nov 12, 2014·International Journal of Systematic and Evolutionary Microbiology·Ryohei NomotoRo Osawa
Apr 5, 2016·Diagnostic Microbiology and Infectious Disease·Velusamy SrinivasanBernard Beall
Jun 28, 2016·Pathogens·Masatoshi OkuraDaisuke Takamatsu
Jan 20, 2017·BMC Research Notes·P ThongkamkoonM Gottschalk
Aug 26, 2017·International Journal of Systematic and Evolutionary Microbiology·Mari TohyaTsutomu Sekizaki

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Citations

Aug 10, 2019·Microbiology Resource Announcements·Nachiko TakeshitaTsutomu Sekizaki
May 30, 2020·Microbiology Resource Announcements·Kasumi Ishida-KurokiTsutomu Sekizaki
May 30, 2020·Microbiology Resource Announcements·Kasumi Ishida-KurokiTsutomu Sekizaki
Jan 8, 2021·BMC Veterinary Research·Nachiko TakeshitaTsutomu Sekizaki

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