Abstract
Delayed platelet engraftment is a well-known complication of umbilical cord blood transplantation (CBT). Megakaryocytes derived from cord blood (CB) in vitro are smaller than megakaryocytes derived from bone marrow (BM) in adults. A small megakaryocyte size might contribute to delayed megakaryocytic maturation. This study included 37 patients undergoing hematopoietic stem cell transplantation (HSCT) at Chang Gung Children's Hospital between July 2011 and June 2013. Blood samples were obtained at different times: preconditioning and post-HSCT days 56 and 97. To test whether platelet activation persists posttransplantation, two commonly used platelet activation marker antibodies, CD62P (P-selectin) and CD42b, were evaluated using whole blood flow cytometry, combining thiazole orange and anti-CD41a staining, to assess reticulated platelets. Serial peripheral blood (PB) samples were obtained posttransplantation from patients undergoing CBT (CBT group; n = 15) and mobilized peripheral blood transplantation (PBT group; n = 22). Platelet activation in the postengraftment samples was considerably higher in the PBT group than the CBT group. Moreover, immature platelet fractions (IPF) were higher in the CBT group. Our results emphasize t...Continue Reading
References
Jun 22, 2000·The New England Journal of Medicine·V RochaE Gluckman
Sep 1, 2001·British Journal of Haematology·R BornsteinC Calés
Jun 17, 2004·British Journal of Haematology·Carol BriggsSamuel J Machin
Oct 29, 2005·Thrombosis Research·Yasunori AbeTsutomu Nobori
Jul 21, 2006·Pathophysiology of Haemostasis and Thrombosis·Alan D Michelson
Sep 5, 2006·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·Marjorie L ZuckerFred V Plapp
Feb 7, 2007·Clinical Rheumatology·Feng WangLing-Quan Tang
Mar 6, 2007·Bone Marrow Transplantation·A TakamiH Asakura
Mar 12, 2008·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Katsuyasu SaigoShunichi Kumagai
May 17, 2008·Transfusion·Joyce CurversPieter F van der Meer
Jan 29, 2009·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Juliet N BarkerAndromachi Scaradavou
May 20, 2009·International Journal of Laboratory Hematology·C Briggs
Mar 11, 2011·Blood·Sarah J BarsamJames B Bussel
Mar 20, 2012·Thrombosis Research·Jeroen F van VelzenWaander L van Heerde
Apr 5, 2012·Clinical Biochemistry·Eva HennelKlas Boeer
Feb 19, 2013·Pediatric Blood & Cancer·Laerke Walther Junggreen HaveMimi Kjaersgaard
Sep 24, 2013·Haematologica·David I MarksMary Eapen
Jan 15, 2014·PloS One·Hong HongRobert W Maitta
Oct 29, 2014·Platelets·Amira Abdel Moneam AdlyMona Mohammed Farahat