Assessment of porcine endogenous retrovirus transmission across an alginate barrier used for the encapsulation of porcine islets

Xenotransplantation
Claire CrossanL Scobie

Abstract

Subcutaneous implantation of a macroencapsulated patch containing human allogenic islets has been successfully used to alleviate type 1 diabetes mellitus (T1DM) in a human recipient without the need for immunosuppression. The use of encapsulated porcine islets to treat T1DM has also been reported. Although no evidence of pathogen transfer using this technology has been reported to date, we deemed it appropriate to determine if the encapsulation technology would prevent the release of virus, in particular, the porcine endogenous retrovirus (PERV). HEK293 (human epithelial kidney) and swine testis (ST) cells were co-cultured with macroencapsulated pig islets embedded in an alginate patch, macroencapsulated PK15 (swine kidney epithelial) cells embedded in an alginate patch and free PK15 cells. Cells and supernatant were harvested at weekly time points from the cultures for up to 60 days and screened for evidence of PERV release using qRT-PCR to detect PERV RNA and SG-PERT to detect reverse transcriptase (RT). No PERV virus, or evidence of PERV replication, was detected in the culture medium of HEK293 or pig cells cultured with encapsulated porcine islets. Increased PERV activity relative to the background was not detected in ST ce...Continue Reading

References

Feb 1, 1971·The Journal of General Virology·J A ArmstrongA T De Madrid
Nov 6, 2004·Journal of Clinical Microbiology·O GarkavenkoR B Elliott
Sep 1, 2005·European Journal of Endocrinology·Rafael A Valdés-GonzálezDavid J G White
Mar 27, 2007·Xenotransplantation·Robert B ElliottChristina Buchanan
Dec 17, 2009·Tissue Engineering. Part a·Sophie VériterDenis Dufrane
Apr 17, 2012·The International Journal of Artificial Organs·Xiao-Lei ShiYi-Tao Ding
Aug 19, 2014·Transplantation Proceedings·S MatsumotoR B Elliott
Oct 25, 2016·Transplantation·David K C CooperEmanuele Cozzi
Nov 5, 2016·Virus Research·Vladimir A MorozovRobert Elliott
Feb 12, 2017·Stem Cells Translational Medicine·Berit L StrandGudmund Skjak-Braek
Dec 9, 2017·Xenotransplantation·Claire CrossanLinda Scobie

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Citations

Jun 19, 2018·Xenotransplantation·Linda ScobiePierre Gianello
Jan 31, 2019·Xenotransplantation·Heather E Levy, Christopher Burlak
Apr 19, 2020·Xenotransplantation·Joachim Denner
Sep 20, 2018·Current Diabetes Reports·Elisabeth KemterEckhard Wolf
Jun 12, 2020·Journal of Tissue Engineering·Jordan MagissonSéverine Sigrist

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