PMID: 2073986Dec 1, 1990

Assessment of precision, concordance, specificity, and sensitivity of islet cell antibody measurement in 41 assays

Diabetologia
Ezio BonifacioG F Bottazzo

Abstract

Forty-one assays were analysed at the 3rd International Workshop on the standardisation of islet cell antibodies. Analysis of precision demonstrated assays consistently detecting blind duplicates within one doubling dilution and capable of discriminating one doubling dilution differences in islet cell antibody concentration. Some assays, however, reported duplicates discrepantly by more than seven doubling dilutions, and consequently could not distinguish even large quantities of islet cell antibodies. Precision was best in assays from laboratories which had participated in all three Standardisation Workshops and was not dependent upon methodology. The use of the Juvenile, Diabetes Foundation reference islet cell antibody standard and standard curves reduced the scatter of results, and was best amongst assays with better precision. Twenty-seven assays reported all ten blood donor sera as negative. However, 14 assays did not, and specificity (negativity in health) was less than 50% in three assays. Low specificity was strongly associated with poor precision. The detection limit of assays ranged from less than 5 to 50 JDF units and was partially dependent upon methodology. Assays incorporating extended incubation had the lowest d...Continue Reading

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Citations

Jun 1, 1991·Diabetes/metabolism Reviews·T J Wilkin
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Jan 23, 1998·Human Immunology·D Dubois-LaforgueS Caillat-Zucman
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Related Concepts

Islet cell surface antibodies
Autoantibodies
Blood Chemical Analysis
Diabetes, Autoimmune
Islets of Langerhans

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