Assessment of ptxD gene as an alternative selectable marker for Agrobacterium-mediated maize transformation

Plant Cell Reports
Hartinio N NahampunKan Wang

Abstract

Bacterial phosphite oxidoreductase gene and chemical phosphite can be used as a selection system for Agrobacterium -mediated maize transformation. Application of phosphite (Phi) on plants can interfere the plant metabolic system leading to stunted growth and lethality. On the other hand, ectopic expression of the ptxD gene in tobacco and Arabidopsis allowed plants to grow in media with Phi as the sole phosphorous source. The phosphite oxidoreductase (PTXD) enzyme catalyzes the conversion of Phi into phosphate (Pi) that can then be metabolized by plants and utilized as their essential phosphorous source. Here we assess an alternative selectable marker based on a bacterial ptxD gene for Agrobacterium-mediated maize transformation. We compared the transformation frequencies of maize using either the ptxD/Phi selection system or a standard herbicide bar/bialaphos selection system. Two maize genotypes, a transformation amenable hybrid Hi II and an inbred B104, were tested. Transgene presence, insertion copy numbers, and ptxD transcript levels were analyzed and compared. This work demonstrates that the ptxD/Phi selection system can be used for Agrobacterium-mediated maize transformation of both type I and type II callus culture and a...Continue Reading

References

Jan 25, 1983·Nucleic Acids Research·M BevanM D Chilton
May 7, 2002·Trends in Plant Science·Mansour KarimiAnn Depicker
Jul 1, 1990·The Plant Cell·W. J. Gordon-KammP. G. Lemaux
Jan 23, 2004·Journal of Biotechnology·Brian Miki, Sylvia McHugh
Jun 23, 2007·Nature Protocols·Yuji IshidaToshihiko Komari
Jul 10, 2008·Plant Physiology·Stephen P Moose, Rita H Mumm
Jan 6, 2011·Methods in Molecular Biology·Bronwyn FrameKan Wang
Sep 17, 2011·Cellular & Molecular Biology Letters·Agata GadaletaAntonio Blanco
Aug 28, 2012·Nature Biotechnology·Damar Lizbeth López-Arredondo, Luis Herrera-Estrella
Dec 1, 1996·Plant Cell Reports·M Joersbo, F T Okkels
Sep 1, 1985·Plant Molecular Biology·P J van den ElzenJ R Bedbrook
May 3, 2014·Journal of Biotechnology·Keisuke KandaAkio Kuroda

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Citations

Nov 20, 2016·The Plant Journal : for Cell and Molecular Biology·Sigrid HeuerHatem Rouached
Oct 28, 2016·Chemical Reviews·Geoff P Horsman, David L Zechel
Aug 5, 2017·Plant Biotechnology Journal·V Mohan M AcharyPawan K Agrawal
Oct 8, 2019·Journal of Environmental Quality·Joseph J Weeks, Ganga M Hettiarachchi
Apr 19, 2019·Molecular Biotechnology·José M Sandoval-VargasJesús A Badillo-Corona
Jul 5, 2018·Proceedings of the National Academy of Sciences of the United States of America·Rafael Catalá, Julio Salinas
Jun 6, 2018·Proceedings of the National Academy of Sciences of the United States of America·Devendra PandeyaKeerti S Rathore
Jun 6, 2020·Frontiers in Plant Science·Marcos Fernando BassoMaria Fatima Grossi-de-Sa
Nov 17, 2017·Plant, Cell & Environment·Kim FeysHilde Nelissen
Apr 9, 2019·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Xueqing DuanHailong An
Oct 26, 2020·Pest Management Science·Elizabeth Dann, Adéle McLeod
Nov 10, 2017·Emerging Topics in Life Sciences·Hui Zhao, Jeffrey D Wolt
Dec 14, 2021·Frontiers in Bioengineering and Biotechnology·Tongtong LiuChuang Wang

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