Assessment of Rhodopseudomonas sphaeroides chromatophore membrane asymmetry through bilateral antiserum adsorption studies.

Journal of Bacteriology
M L CollinsR A Niederman

Abstract

The asymmetric structure of the Rhodopseudomonas sphaeroides chromatophore membrane was examined in detail by crossed immunoelectrophoresis techniques. Because these methods are quantitative and allow increased resolution and sensitivity, it was possible to analyze simultaneously the relative transmembrane distribution of a number of previously identified antigenic components. This was demonstrated by analysis of immunoglobulin samples that were adsorbed by preincubation with either isolated chromatophores or osmotically protected spheroplasts. The photochemical reaction center, the light-harvesting bacteriochlorophyll a-protein complex, the L-lactate dehydrogenase, and reduced nicotinamide adenine dinucleotide dehydrogenase (EC 1.6.99.3) were found to be exposed on the chromatophore surface (cytoplasmic aspect of the membrane within the cell). Other antigenic components were found to be exposed on the surface of spheroplasts (periplasmic aspect of the in vivo chromatophore membrane). Antigens with determinants expressed on both sides of the chromatophore membrane were also identified. Charge shift crossed immunoelectrophoresis confirmed the suggested amphiphilic character of the pigment-protein complexes and identified several...Continue Reading

References

Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·P Owen, M R Salton
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P Owen, H R Kaback
Apr 17, 1979·Biochemistry·P Owen, H R Kaback
Apr 17, 1979·Biochemistry·P Owen, H R Kaback
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A Helenius, K Simons
Feb 25, 1977·Science·J E Rothman, J Lenard
Apr 1, 1976·Infection and Immunity·C J SmythM R Salton
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·N Harboe, A Ingild
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·B Weeke
Apr 1, 1969·European Journal of Biochemistry·P ScholesJ Moyle
Feb 1, 1957·Journal of Cellular Physiology·G COHEN-BAZIRER Y STANIER
Feb 1, 1963·The Journal of Cell Biology·G COHEN-BAZIRE, R KUNISAWA
May 1, 1965·Journal of Bacteriology·S C HOLT, A G MARR

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Citations

Mar 1, 1983·Journal of Bacteriology·P R Mueller, M L Collins

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