Assessment of sample handling practices on microbial activity in sputum samples from patients with cystic fibrosis

Letters in Applied Microbiology
A NelsonS P Cummings

Abstract

The aim of this study was to quantitatively and qualitatively assess the effect of sample storage on the metabolically active microbial community found in sputum samples from patients with cystic fibrosis (CF). Sputum samples were collected and split in two equal aliquots one of which was immersed in RNAlater and refrigerated immediately, the second stored at room temperature for 24 h and RNAlater was subsequently added. mRNA was extracted, and RT-PCR-DGGE and qPCR analysis of the bacterial and fungal communities was carried out. Significant differences in the bacterial communities between the two protocols were observed but there were no significant difference seen in the fungal community analyses. Analysis by qPCR demonstrated that room temperature storage gave statistically significant increases in eubacteria and Pseudomonas spp. and a statistically significant decrease in those of Haemophilus influenzae. The analysis of metabolically active microbial communities from CF sputum using molecular techniques indicated that samples should be stored at 4 degrees C upon addition of RNAlater to obtain an accurate depiction of the CF lung microbiota. Also, storing respiratory samples at room temperature may cause an over representati...Continue Reading

References

Dec 1, 1990·Journal of Medical Microbiology·R J van KetelL van Alphen
Nov 1, 1995·Journal of Clinical Microbiology·G S SandhuG D Roberts
Aug 1, 1996·Journal of Clinical Pathology·F K GouldP R Sisson
Feb 3, 2007·Journal of Clinical Microbiology·Nabeel RishmawiMusa Y Hindiyeh
Jun 29, 2007·Journal of Clinical Microbiology·F DalwaiJ Pratten
Sep 26, 2007·Journal of Clinical Pathology·A PyeR A Stockley
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·J Kirk HarrisNorman R Pace
Jan 26, 2008·Journal of Applied Microbiology·J Baxter, S P Cummings
Feb 12, 2008·American Journal of Respiratory and Critical Care Medicine·Michael M TunneyJ Stuart Elborn
Sep 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Christopher D SibleyMichael G Surette

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Citations

Feb 22, 2013·Archives of Disease in Childhood. Fetal and Neonatal Edition·Christopher James StewartJanet Elizabeth Berrington
Aug 1, 2013·Genome Medicine·Lijia CuiElodie Ghedin
Feb 20, 2016·Early Human Development·Bashir AbdulkadirChristopher J Stewart
Jun 12, 2012·Translational Research : the Journal of Laboratory and Clinical Medicine·James M BeckGary B Huffnagle
Apr 25, 2014·Journal of Clinical Microbiology·Joshua R StokellTodd R Steck
Sep 12, 2012·Current Opinion in Pulmonary Medicine·Harvey R Rabin, Michael G Surette
Apr 22, 2017·FEMS Microbiology Reviews·Chloe E HuseyinPauline D Scanlan

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