Assessment of sex chromosome ratio and aneuploidy rate in motile spermatozoa selected by three different methods

Human Reproduction
O SamuraK Ohama

Abstract

Using three-colour fluorescence in-situ hybridization, sex chromosome ratios and frequencies of diploidy and disomy for chromosomes X, Y and 18 were compared in spermatozoa of good and poor motility after separation by swim-up, glass-wool and two-layer discontinuous Percoll methods. Semen samples were collected from seven normal males aged 26-31 years. A minimum of 6000 sperm nuclei per sample were evaluated for each chromosome for a total of 308,432 sperm nuclei. Hybridization efficiency was 99.8%. A slight change in the ratio of X- to Y-bearing spermatozoa was noted after Percoll separation (from 49.3:49.5 to 50.0:48.9; P = 0.036 and P = 0.046), but not after separation by the other two methods. We did not observe significant differences in the disomy rates for sex chromosomes or chromosome 18 or in the diploidy rate between spermatozoa with good and poor motility after separation by any of the three methods. Our data indicate that separation of motile spermatozoa does not alter the ratio of X- to Y-bearing spermatozoa to a degree that represents sex chromosome selection.

Citations

Mar 4, 1999·The Journal of Obstetrics and Gynaecology Research·P Li, H Hoshiai
Dec 9, 2003·Archives of Andrology·K S BalliP J Chan
Jul 10, 2001·Archives of Andrology·J M Nani, R S Jeyendran
May 23, 2007·Fertility and Sterility·Eric Gonzalez-MerinoSerena Emiliani
Mar 30, 2001·Human Reproduction·Y OhashiK Ohama
Jul 19, 2019·Biology of Reproduction·Lorena RodrigoCarmen Rubio
Feb 11, 2020·Frontiers in Cell and Developmental Biology·Md Saidur Rahman, Myung-Geol Pang

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