PMID: 9530870Apr 8, 1998Paper

Assessment of stress fiber orientation during healing of radial keratotomy wounds using confocal microscopy

Scanning
W Matthew PetrollJ V Jester

Abstract

Radial keratotomy (RK) is a refractive surgical procedure in which partial thickness incisions are made in the cornea in order to alter its shape. Previous studies suggest that RK wounds undergo changes in wound gape in response to the ingrowth of myofibroblasts which mediate subsequent wound contraction and may modulate changes in corneal curvature seen after RK. A recent quantitative analysis of f-actin organization in full-thickness incisional wounds in the rabbit demonstrated that microfilament bundles (stress fibers) present in myofibroblasts align parallel to the long axis of the wound during wound contraction. To investigate whether the same pattern of alignment occurs after RK, a similar analysis of f-actin organization was undertaken using the cat RK model. Radial keratotomy wounds were studied from 10 to 28 days after surgery using en block staining with fluorescein isothiocyanate (FITC) phalloidin, and three-dimensional (3-D) datasets (z-series of en face optical sections) were collected using laser confocal microscopy at various regions within the wound. In addition, conventional en face sections were double-labeled using combinations of phalloidin and antibodies to fibronectin and alpha 5 beta 1 integrin. Myofibrob...Continue Reading

References

Jan 1, 1991·Connective Tissue Research·P L Kronick, M S Sacks
Jul 1, 1987·IEEE Transactions on Bio-medical Engineering·S ChaudhuriC B Frank
Apr 1, 1972·The Journal of Experimental Medicine·G GabbianiG Majno
Aug 1, 1995·The British Journal of Ophthalmology·G R MellesN SundarRaj
Oct 1, 1994·Archives of Ophthalmology·G O WaringP J McDonnell

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Citations

May 29, 2007·Journal of Cataract and Refractive Surgery·Lana J NagyKrystel R Huxlin
May 20, 2015·Journal of Esthetic and Restorative Dentistry : Official Publication of the American Academy of Esthetic Dentistry ... [et Al.]·Neelesh SinghDilip Naik
Dec 9, 2008·Cell Motility and the Cytoskeleton·Dimitris KaramichosW Matthew Petroll
Feb 6, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·J R RalphsM Benjamin
Jun 9, 2004·Vision Research·Krystel R HuxlinDavid Williams
Sep 19, 2006·Experimental Cell Research·Areum KimW Matthew Petroll

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