Assessment of the Cavidi ExaVir Load Assay for Monitoring Plasma Viral Load in HIV-2-Infected Patients

Journal of Clinical Microbiology
Pedro BorregoNuno Taveira

Abstract

HIV plasma viral load is an established marker of disease progression and of response to antiretroviral therapy, but currently there is no commercial assay validated for the quantification of viral load in HIV-2-infected individuals. We sought to make the first clinical evaluation of Cavidi ExaVir Load (version 3) in HIV-2-infected patients. Samples were collected from a total of 102 individuals living in Cape Verde, and the HIV-2 viral load was quantified by both ExaVir Load and a reference in-house real-time quantitative PCR (qPCR) used in Portugal in 91 samples. The associations between viral load and clinical prognostic variables (CD4+ T cell counts and antiretroviral therapy status) were similar for measurements obtained using ExaVir Load and qPCR. There was no difference between the two methods in the capacity to discriminate between nonquantifiable and quantifiable HIV-2 in the plasma. In samples with an HIV-2 viral load quantifiable by both methods (n = 27), the measurements were highly correlated (Pearson r = 0.908), but the ExaVir Load values were systematically higher relative to those determined by qPCR (median difference, 0.942 log10 copies/ml). A regression model was derived that enables the conversion of ExaVir L...Continue Reading

References

Jan 1, 1990·Journal of Clinical Epidemiology·D V Cicchetti, A R Feinstein
Nov 1, 1983·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·H Passing, Bablok
Feb 1, 1997·Clinical and Experimental Pharmacology & Physiology·J Ludbrook
Feb 13, 2001·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·G MuyldermansS Lauwers
Jun 13, 2002·Clinical and Experimental Pharmacology & Physiology·John Ludbrook
May 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Philippe LemeyAnne-Mieke Vandamme
Apr 28, 2005·Current HIV Research·Vicki L GreengrassSuzanne M Crowe
May 20, 2005·Journal of Medical Virology·Anders MalmstenJ Simon Gronowitz
Oct 28, 2005·Current HIV Research·Elizabeth SeyoumSven Britton
Dec 8, 2005·Journal of Clinical Microbiology·Cheryl JenningsJames W Bremer
Oct 13, 2006·PLoS Medicine·Susan A FiscusUNKNOWN Forum for Collaborative HIV Research Alternative Viral Load Assay Working Group
Aug 10, 2007·Journal of Virological Methods·Kim SteegenMarleen Temmerman
Apr 25, 2008·Journal of Clinical Microbiology·Florence DamondUNKNOWN ACHIEV2E Collaboration on HIV-2 Infection Study Group
Jul 17, 2009·Journal of Clinical Microbiology·Vicki L GreengrassSuzanne M Crowe
Jul 21, 2009·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Vicki GreengrassSuzanne M Crowe
Nov 10, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Sonia NapravnikSusan A Fiscus
Nov 11, 2009·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Lisette B van RooijenSuzanne M Crowe
Jun 25, 2010·Journal of Clinical Microbiology·Anna Rita CiccaglioneLeonardo Palombi
Sep 14, 2010·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Paul StewartSusan A Fiscus
Sep 18, 2010·Diagnostic Microbiology and Infectious Disease·Dedong HuangYongtao Sun
Mar 4, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Omobolaji T Campbell-Yesufu, Rajesh T Gandhi
Aug 5, 2011·Journal of Clinical Microbiology·F DamondUNKNOWN ACHI(E)V(2E) Study Group
Jul 27, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Ming ChangRobert W Coombs
Sep 15, 2012·The Journal of Antimicrobial Chemotherapy·Joana Cavaco-SilvaUNKNOWN Portuguese HIV-2 Resistance Study Group
Oct 30, 2012·International Journal of STD & AIDS·K HeitzingerUNKNOWN University of Washington-Dakar HIV and Cervical Cancer Study Group
Aug 24, 2013·International Journal of STD & AIDS·Sarah PeitzmeierStefan Baral
Sep 7, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Séverine DelarueFrançois Simon
Dec 18, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Linda M StyerMonica M Parker
Feb 22, 2014·PloS One·Kimberly A SollisRosanna W Peeling
Jun 13, 2014·Journal of Clinical Microbiology·Véronique Avettand-FenoelUNKNOWN ANRS-CO5 HIV-2 and the ANRS-AC11 Quantification Working Group
Jun 18, 2015·Journal of Medical Virology·Agnès Gautheret-DejeanFrancis Barin
Jun 26, 2015·PloS One·Didier K EkouéviUNKNOWN IeDEA West Africa collaboration

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.