Assessment of the cell-mediated immune response in chickens by detection of chicken interferon-gamma in response to mitogen and recall Newcastle disease viral antigen stimulation

Avian Pathology : Journal of the W.V.P.A
Bénédicte LambrechtThierry van den Berg

Abstract

The potential of a capture enzyme-linked immunosorbent assay (ELISA) specific for chicken interferon-gamma (ChIFN-gamma) has been evaluated as a tool to assess cell-mediated immunity (CMI) in the chicken. In a first step, ChIFN-gamma production and cell proliferation of mitogen-activated chicken splenocytes have been compared. In general, for each of the stimulation conditions where significant proliferation was observed, production of ChIFN-gamma could be measured by ELISA. In our hands, the combination of ionomycin and phorbol-12-myristate 13-acetate or the use of recombinant chicken interleukin-2 gave the most satisfactory results. Then, the CMI response induced by live or killed Newcastle disease virus (NDV) vaccines has been evaluated sequentially by ex vivo antigen-specific ChIFN-gamma production and cell proliferation of splenocytes from immune chickens. The ex vivo data showed that both types of NDV vaccines are capable of stimulating CMI responses to NDV in chickens as measured by the ChIFN-gamma ELISA. However, most of the chickens vaccinated with the live vaccine produced ChIFN-gamma after antigen recall stimulation, from 2 to 4 weeks after vaccination, when only some chickens vaccinated with the inactivated vaccine ...Continue Reading

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Citations

Sep 28, 2007·Avian Pathology : Journal of the W.V.P.A·Fabienne RauwThierry van den Berg
Nov 26, 2009·Avian Pathology : Journal of the W.V.P.A·Dennis Rubbenstroth, Silke Rautenschlein
Feb 24, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Fabienne RauwThierry van den Berg
Jul 15, 2015·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Bin JiPei-Fu Chen
Nov 16, 2007·International Journal of Immunogenetics·K A AhmedT J Rasool
Feb 22, 2011·Scandinavian Journal of Immunology·L R NorupH R Juul-Madsen
Aug 28, 2018·Comparative Clinical Pathology·Hadi RohollahzadehAmir Hossein Asl Najjari
Jul 8, 2020·BMC Veterinary Research·Karla Lucía F AlvarezManolo Fernández-Díaz
May 19, 2019·Cellular and Molecular Life Sciences : CMLS·Manman DaiMing Liao
Feb 20, 2021·Comparative Immunology, Microbiology and Infectious Diseases·Maryam BarkhordariShahla Shahsavandi
May 10, 2021·Veterinary Immunology and Immunopathology·Nathalie GuriecPi Nyvall Collén
Jun 28, 2019·Developmental and Comparative Immunology·Marina DobnerSilke Rautenschlein

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Methods Mentioned

BETA
enzyme-linked immunosorbent assays
ELISA
protein assay
PMA
FACS
glycosylation

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