Assessment of the cellular localisation of the annexin A2/S100A10 complex in human placenta

Journal of Molecular Histology
Seham A Abd El-Aleem, Lodewijk V Dekker

Abstract

The AnxA2/S100A10 complex has been implicated in various placental functions but although the localisation of these proteins individually has been studied, there is no information about the localisation of their complex in situ at the cellular level. Using the proximity ligation technique, we have investigated the in situ localisation of AnxA2/S100A10 complex in the placenta and have compared this with the location patterns of the individual proteins. High levels of expression of AnxA2/S100A10 complexes were observed in the amniotic membrane and in blood vessel endothelial cells. Lower levels were detected in the brush border area of the syncytium and in the trophoblasts. Immunohistochemical analysis of AnxA2 and S100A10 individually revealed broadly similar patterns of localisation. The brush border staining pattern suggests that in this location at least some of the AnxA2 is not in complex with S100A10. The formal location of the AnxA2/S100A10 complex is compatible with a role in cell-cell interaction, intracellular transport and secretory processes and regulation of cell surface proteases, implying contributions to membrane integrity, nutrient exchange, placentation and vascular remodelling in different parts of the placenta...Continue Reading

References

Sep 1, 1996·American Journal of Reproductive Immunology : AJRI·E K Kristoffersen, R Matre
Feb 1, 1997·American Journal of Reproductive Immunology : AJRI·A AarliR Matre
Jan 14, 1999·Nature Structural Biology·S RétyA Lewit-Bentley
Jan 1, 2004·The Journal of Clinical Investigation·Qi LingKatherine A Hajjar
Apr 29, 2004·American Journal of Physiology. Renal Physiology·David B N LeeIrene L Hale
Aug 11, 2004·The Journal of Biological Chemistry·Arunkumar B DeoraKatherine A Hajjar
Sep 17, 2004·The Journal of Clinical Investigation·Kristy Red-HorseSusan J Fisher
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Volker GerkeStephen E Moss
Aug 18, 2006·Cell Calcium·Katia MonastyrskayaAnnette Draeger
Dec 11, 2007·The Journal of Biological Chemistry·Wen TianWonhwa Cho
Apr 25, 2008·The Journal of Biological Chemistry·Kai-Li HeKatherine A Hajjar
Aug 15, 2008·American Journal of Physiology. Renal Physiology·David B N LeeHarry J Ward
Nov 22, 2008·Methods in Enzymology·Nathan M Hunkapiller, Susan J Fisher
Nov 9, 2010·Best Practice & Research. Clinical Obstetrics & Gynaecology·Anthony M Carter, Robert Pijnenborg
Jul 20, 2011·Blood·Alexi P SuretteDavid M Waisman
Sep 13, 2011·Blood·Patricia A MadureiraDavid M Waisman
May 18, 2012·Cell and Tissue Research·A C MamedeM F Botelho
Nov 3, 2012·Journal of Biomedicine & Biotechnology·Patricia A MadureiraDavid M Waisman
Mar 14, 2013·Seminars in Thrombosis and Hemostasis·Min Luo, Katherine A Hajjar
Oct 11, 2014·British Journal of Pharmacology·Yidong LiuLodewijk V Dekker
Jan 14, 2016·Cell Adhesion & Migration·Laurence BissonnetteSamir Hamamah

❮ Previous
Next ❯

Citations

Dec 24, 2018·International Journal of Molecular Sciences·Tannith M NoyeCarmela Ricciardelli
Oct 15, 2021·Laboratory Investigation; a Journal of Technical Methods and Pathology·Xiaoxia BaiYongmei Xi

❮ Previous
Next ❯

Methods Mentioned

BETA
proximity ligation
light microscopy

Software Mentioned

Adobe Photoshop

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Annals of the New York Academy of Sciences
J A Hardin, D G Gall
Advances in Experimental Medicine and Biology
M G BrunetteS Allard
Mineral and Electrolyte Metabolism
T P Dousa, S A Kempson
Biochimica Et Biophysica Acta
P Truman, H C Ford
© 2022 Meta ULC. All rights reserved