Assessment of the Cytosensor Microphysiometer Assay in the COLIPA In Vitro Eye Irritation Validation Study

Toxicology in Vitro : an International Journal Published in Association with BIBRA
John W HarbellA Peterson

Abstract

The Cytosensor(TM) microphysiometer assay and its associated prediction model were evaluated in the COLIPA ocular irritation validation study for cosmetic ingredients and formulations. Test materials were prepared in low-buffer medium and exposed to L929 cells grown in transwells. The metabolic rate of the cell population was measured after each dose and the dose inducing a 50% decrease in the rate (MRD(50)) was determined and used to predict the ocular irritation potential. Only 29 of the 55 materials could be tested because of solubility limitations. The irritancy potential of many chemical classes was underpredicted by the assay, particularly acids, bases and organics. The use of the assay for surfactants and surfactant-based formulations showed promise, confirming the use of the method for these types of materials, although some revision of the prediction model would be necessary. Excellent interlaboratory reproducibility for the MRD(50) values across all test materials was observed.

References

Jul 1, 1991·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·L H BrunerR D Parker
Jan 1, 1996·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S D GettingsJ Zyracki
Jan 1, 1997·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J W HarbellD Acosta
Jan 1, 1997·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J BradlawN Wilcox
Dec 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M BallsH Spielmann
Feb 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·P G BrantomM Stern
Aug 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A RougierK G Dossou
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S J ReaderM Balls
Jan 1, 1991·Toxicology in Vitro : an International Journal Published in Association with BIBRA·L H BrunerV C Muir
Jul 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·D M BagleyA P Walker
Jul 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K A WallaceR D Curren
Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·P CatrouxM Cottin

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Citations

Aug 19, 2007·Journal of Pharmaceutical Sciences·M P Vinardell, M Mitjans
Aug 21, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M EngelkeK R Mewes
Apr 28, 2009·Regulatory Toxicology and Pharmacology : RTP·Pauline McNameeJulia Scheel
Dec 15, 2015·International Journal of Toxicology·Palma Ann MaroneA Wallace Hayes
Aug 12, 2014·Current Protocols in Toxicology·Jennifer R NashRodger Curren
Dec 17, 2008·Alternatives to Laboratory Animals : ATLA·Christina GrindonJohn F Garrod
Mar 13, 2008·Alternatives to Laboratory Animals : ATLA·Christina GrindonJohn F Garrod
Dec 15, 2004·Alternatives to Laboratory Animals : ATLA·Ludwig A Hothorn
Oct 1, 2005·Alternatives to Laboratory Animals : ATLA·Chantra EskesValérie Zuang

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