Assessment of the effects of graphene exposure in Danio rerio: A molecular, biochemical and histological approach to investigating mechanisms of toxicity

Chemosphere
Amanda Lucena FernandesJuliane Ventura-Lima

Abstract

Graphene has been shown to induce toxicity in mammals and marine crustaceans; however, information regarding oxidative stress in fish is scarce. The aim of this study was to evaluate the mechanism of graphene toxicity in different tissues of Danio rerio, considering different parameters of stress. Animals were injected intraperitoneally (i.p.) with 10 μL of suspensions containing different graphene concentrations (5 and 50 mg/L); the gills, intestine, muscle and brain were analysed 48 h later. There was no significant difference in the expression of the gclc (glutamate cysteine ligase catalytic subunit) and nrf2 (nuclear factor (erythroid-derived 2)-like 2) genes after exposure. In contrast, glutamate cysteine ligase (GCL) and glutathione-S-transferase (GST) activities were modulated and the glutathione (GSH) concentration was reduced in different tissues and at different concentrations. Lipid damage was observed in the gills. Histological analyses were performed to observe if the exposure could induce pathological damage in these tissues. The results showed pathological effects in all tissues, excluding the intestine, after exposure to both concentrations. Overall, these results indicate that graphene induces different grades ...Continue Reading

Citations

Jul 7, 2019·Fish Physiology and Biochemistry·Jaqueline P SouzaValtencir Zucolotto
Mar 4, 2021·Environmental Science and Pollution Research International·Bangeppagari ManjunathaSang Joon Lee
Feb 23, 2021·The Science of the Total Environment·Ignacio Martínez-ÁlvarezAmaia Orbea

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