Assessment of the electrochemical effects of pulsed electric fields in a biological cell suspension

Bioelectrochemistry
Djamel Eddine ChafaiDamijan Miklavčič

Abstract

Electroporation of cells is successfully used in biology, biotechnology and medicine. Practical problems still arise in the electroporation of cells in suspension. For example, the determination of cell electroporation is still a demanding and time-consuming task. Electric pulses also cause contamination of the solution by the metal released from the electrodes and create local enhancements of the electric field, leading to the occurrence of electrochemical reactions at the electrode/electrolyte interface. In our study, we investigated the possibility of assessing modifications to the cell environment caused by pulsed electric fields using electrochemical impedance spectroscopy. We designed an experimental protocol to elucidate the mechanism by which a pulsed electric field affects the electrode state in relation to different electrolyte conductivities at the interface. The results show that a pulsed electric field affects electrodes and its degree depends on the electrolyte conductivity. Evolution of the electrochemical reaction rate depends on the initial free charges and those generated by the pulsed electric field. In the presence of biological cells, the initial free charges in the medium are reduced. The electrical curren...Continue Reading

References

Jan 1, 1992·Annals of Biomedical Engineering·J R Macdonald
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May 11, 2004·IEEE Transactions on Bio-medical Engineering·Rafael V DavalosBoris Rubinsky
Mar 29, 2005·Biophysical Journal·Mojca PavlinDamijan Miklavcic
Jan 30, 2007·Bioelectrochemistry·David CukjatiLluis M Mir
Feb 5, 2008·Biosensors & Bioelectronics·Jeong Ah KimJun Keun Chang
Jul 21, 2010·The Journal of Membrane Biology·Igor MarjanovicMojca Pavlin
Jan 1, 2006·CSH Protocols·Andras NagyRichard Behringer
Sep 22, 2012·IEEE Transactions on Bio-medical Engineering·Airton RamosJefferson L B Marques
Jun 7, 2014·Annual Review of Biomedical Engineering·Martin L YarmushDamijan Miklavčič
Oct 8, 2014·The Journal of Membrane Biology·Samo Mahnič-KalamizaDamijan Miklavčič

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Citations

Mar 8, 2016·Bioelectrochemistry·Tina Batista NapotnikDamijan Miklavčič
Feb 7, 2019·In Vitro Cellular & Developmental Biology. Animal·Min Seong KimSeung Tae Lee
May 28, 2020·Advanced Biosystems·Djamel Eddine ChafaiPavel Dráber
Oct 21, 2018·Scientific Reports·Paulius RuzgysSaulius Šatkauskas
Apr 30, 2016·Biotechnology for Biofuels·Alexander GolbergWolfgang Frey
May 12, 2021·Bioelectrochemistry·Tjaša PotočnikAlenka Maček Lebar
Jul 28, 2018·Chemical Reviews·Martin P StewartKlavs F Jensen

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