Assessment of the FasciMol-ELISA in the detection of the trematode Fasciola hepatica in field-collected Galba cubensis: a novel tool for the malacological survey of fasciolosis transmission

Parasites & Vectors
Annia AlbaJorge Sarracent

Abstract

Fasciolosis is one of the food-borne neglected trematodioses that has reemerged as a human disease while its effects on domestic animal health remains of significant economic consideration. Being snail-borne disease, the accurate and time-saving epidemiological surveillance of the transmission foci where infected lymnaeid snails occur could be essential to effectively focus or redirect control strategies. For this purpose, the first monoclonal antibody-based immunoenzymatic assay to detect Fasciola hepatica-infected snails (FasciMol-ELISA) was recently developed and showed a high sensitivity and specificity when tested in an experimental F. hepatica - Galba cubensis system. Here, we surveyed populations of G. cubensis occurring in western Cuba for the assessment of the FasciMol-ELISA in determining natural F. hepatica infection in this intermediate host. A multiplex PCR, previously developed to detect F. hepatica in G. cubensis, was used for sample classification. Snail dissection method was also employed as screening technique. A Χ(2) test and a Kappa index were calculated to evaluate the positivity and the level of agreement between the FasciMol-ELISA and the snail dissection methods with the multiplex PCR, respectively. Galb...Continue Reading

References

Feb 19, 1998·International Journal for Parasitology·R M KaplanC H Courtney
Jan 24, 2006·Veterinary Parasitology·Marcela A CucherCristina Wisnivesky-Colli
Feb 3, 2007·Veterinary Parasitology·Florencia KleimanCristina Wisnivesky-Colli
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Jan 19, 2015·Veterinary Parasitology·Gabriela Knubben-Schweizer, Paul R Torgerson

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Methods Mentioned

BETA
ELISA
PCR
dissection

Software Mentioned

FasciMol
MapInfo

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