Assessment of the in vitro and in vivo biological activities of the human follicle-stimulating isohormones

Molecular and Cellular Endocrinology
Jorgelina Barrios-de-TomasiA Ulloa-Aguirre

Abstract

Gonadotropins are synthesized and released in different molecular forms. In this article, we present evidence that the glycosylation variants of human pituitary FSH exhibit differential and divergent effects at the target cell level and that less sialylated, short-lived variants may exert significant effects in in vivo conditions. Less acidic/sialylated glycoforms (elution pH value 6.60-4.60 as disclosed by high resolution chromatofocusing of anterior glycoprotein extracts), induced higher cAMP release, estrogen production and tissue-type plasminogen activator (tPA) enzyme activity as well as cytochrome P450 aromatase and tPA mRNA expression in cultured rat granulosa cells than the more acidic analogs (pH<4.76). By contrast, the more acidic/sialylated glycoforms induced higher alpha-inhibin subunit mRNA expression than their less acidic counterparts. In cumulus enclosed oocytes isolated from mice ovaries, addition of less acidic isoforms induced resumption of meiosis more efficiently than the more acidic analogs. Interestingly, the least acidic isoform (pH>7.10) behave as a strong antagonist of several FSH-mediated effects. Assessment of the in vivo effects of the isoforms on granulosa cell proliferation in follicles from immat...Continue Reading

References

Mar 15, 1985·Biochemical and Biophysical Research Communications·M HattoriK Wakabayashi
Oct 1, 1994·Journal of Molecular Endocrinology·G MedriC Ronin
Dec 20, 1996·Molecular and Cellular Endocrinology·P G StantonD M Robertson
May 1, 1996·Molecular Human Reproduction·R de LeeuwL Kloosterboer
Nov 5, 1997·Molecular and Cellular Endocrinology·L SchaafL D Kohn
Jul 28, 1999·Molecular Human Reproduction·C Yding AndersenA G Byskov
Jan 5, 2000·Clinical Endocrinology·R L OliverW R Robertson
Apr 29, 2000·Endocrine·A Ulloa-AguirreJ A Dias
May 16, 2000·The Journal of Endocrinology·C M TimossiA Ulloa-Aguirre

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Citations

Jul 9, 2011·Reviews in Endocrine & Metabolic Disorders·George R Bousfield, James A Dias
Jun 2, 2006·Reproduction, Fertility, and Development·E ArrietaG Perera-Marín
May 9, 2009·BMC Cell Biology·Jeroen R P M Strating, Gerard J M Martens
Jan 9, 2010·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Ricardo J Solá, Kai Griebenow
Mar 30, 2011·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Kwang Seok SeoJai Myung Yang
Mar 1, 2014·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Joan-Carles ArceAntonio La Marca
May 11, 2010·Journal of Reproductive Immunology·Fortunata CarboneGiuseppe Matarese
May 20, 2005·Reproductive Biomedicine Online·Claudio WolfensonSonja Jespersen
Apr 17, 2007·Animal Reproduction Science·G Perera-MarínE González-Padilla
Jun 9, 2004·Biochemical and Biophysical Research Communications·Panayiotis E StevisDarlene C Deecher
Mar 4, 2011·Reproduction in Domestic Animals = Zuchthygiene·M W HesserJ R Gibbons
Aug 26, 2009·International Journal of Andrology·D A Adamopoulos, E G Koukkou
Feb 22, 2005·Animal Reproduction Science·G Perera-MarínE González-Padilla
Dec 29, 2004·Domestic Animal Endocrinology·M TirelliC Tamanini
Jun 16, 2009·General and Comparative Endocrinology·Gen HiyamaDavid Zadworny
Oct 20, 2006·Journal of Chromatography. a·Renan Fernandes LoureiroMaria Teresa C P Ribela
Sep 12, 2006·Animal Reproduction Science·S Rojas-MayaG Perera-Marín
Apr 27, 2004·Journal of Pharmacological Sciences·Minoru HatsutaHiroshi Kogo
Oct 19, 2016·Molecular Pharmacology·Alfredo Ulloa-Aguirre, Teresa Zariñán
Feb 14, 2018·Journal of Molecular Endocrinology·Nandana Das, T Rajendra Kumar
Jul 10, 2018·Endocrinology·Alfredo Ulloa-AguirrePascale Crépieux
Jul 5, 2003·The Journal of Clinical Endocrinology and Metabolism·Signe PerlmanHans T Schambye
May 29, 2004·The Journal of Pharmacology and Experimental Therapeutics·Nura O ElmagbariEdward J Bilsky
Aug 10, 2006·The Journal of Clinical Endocrinology and Metabolism·Claus Yding Andersen, Anne Grete Byskov
Jul 10, 2019·The Journal of Clinical Endocrinology and Metabolism·Stine Gry KristensenClaus Yding Andersen
Apr 29, 2020·Journal of the Endocrine Society·Teresa ZariñánAlfredo Ulloa-Aguirre
Nov 17, 2020·Toxicology and Industrial Health·Acikgoz BurcuKiray Muge
Jun 3, 2006·Reproductive Biomedicine Online·J Barrios-de-TomasiA Ulloa-Aguirre

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