Assessment of the quantitative determination of paralytic shellfish poisoning toxins by pre-column derivatization and elimination of interfering compounds by solid-phase extraction

Food Additives and Contaminants
P Vale, Hamid Taleb

Abstract

Monitoring programmes for paralytic shellfish poisoning toxins in bivalve molluscs still rely heavily on the use of mouse bioassays (MBA) for consumer protection. A high-performance liquid chromatography (HPLC) methodology (Lawrence method) was implemented in 1996 in the Portuguese monitoring programme as a complementary means of analysis. Comparison between MBA and HPLC was done at the time only by a qualitative approach due to the scarce number of positive samples tested. More quantitative data were obtained recently when studying toxin profiles in Moroccan shellfish, and the correlation found between these two methodologies is reported here for the first time. Two different matrices were studied: blue mussel and the giant cockle Acanthocardia tuberculatum. A good linear correlation was obtained for both matrices. However, a second-degree polynomial best fitted the data at both low and high extremes of toxicity. According to the HPLC quantitative results, 13% of false-negatives could be obtained by MBA due to an underestimation of toxicity near the limit of detection of the MBA. Difficulties on relying solely on HPLC for consumer protection have been aroused with uncommon matrices, such as imported clams or crustaceans, due t...Continue Reading

References

Mar 1, 1991·Zentralblatt für Veterinärmedizin. Reihe A·G Sandh
Sep 25, 1998·Journal of Neurology·M de CarvalhoJ de Sá
Oct 12, 2000·Toxicon : Official Journal of the International Society on Toxinology·P Vale, M A de M Sampayo
Oct 16, 2001·Toxicon : Official Journal of the International Society on Toxinology·H TalebM Blaghen
Feb 1, 2002·Toxicon : Official Journal of the International Society on Toxinology·Paulo Vale, Maria Antónia de M Sampayo
Oct 9, 2002·Toxicon : Official Journal of the International Society on Toxinology·Joanne F JellettRichard E Barrett
Feb 5, 2003·Toxicon : Official Journal of the International Society on Toxinology·Hamid TalebMohammed Blaghen
Jul 18, 2003·Ophthalmology·Omar M Durrani, Omar M Durani
Jul 25, 2003·Journal of Chromatography. a·Michael A Quilliam
Nov 8, 2003·Analytical and Bioanalytical Chemistry·S Effkemann, F Feldhusen
Mar 23, 2004·Toxicon : Official Journal of the International Society on Toxinology·T N AspT Aune
Jun 19, 2004·Food Additives and Contaminants·H P Van EgmondH J Van den Top
Sep 20, 2005·Toxicon : Official Journal of the International Society on Toxinology·Reqia SagouMohamed Loutfi

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HPLC ‘ Chemstation
HP Chemstation

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