Assessment of the reliability or amplitude histograms from excitatory synapses in rat hippocampal CA1 in vitro

The Journal of Physiology
A U LarkmanK J Stratford

Abstract

1. Excitatory postsynaptic potentials (EPSPs) were evoked using minimal extracellular stimulation and recorded from pyramidal cells from the CA1 region of slices taken from adult rats and maintained in vitro. 2. Segments of data were selected that gave EPSP amplitude frequency histograms that showed approximately equally spaced peaks. Selection was performed either on the basis of stationarity of the EPSP mean and standard deviation, or on the trajectory of a graph of the (coefficient of variation)-2 against mean for the EPSP, or, in some cases, by trial and error. 3. For each histogram, we determined the likelihood that peaks of similar sharpness and equality of spacing could have arisen by sampling artifact from a smooth distribution, using a method based on autocorrelation and Monte Carlo simulation. 4. Thirty-three histograms were analysed. For twenty-six of these, the likelihood of sampling artifact was estimated at 1 in 100 or less, and for eleven histograms the likelihood was less than 1 in 1000. For the histogram with the clearest peaks, the likelihood was less than 1 in 350,000. Histograms judged to be reliable by this method could occur when the EPSP mean amplitude was changing. 5. We conclude that random sampling art...Continue Reading

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Citations

Dec 12, 2003·Journal of Neuroscience Methods·John M Bekkers
Dec 12, 2003·Journal of Neuroscience Methods·Christian Stricker, Stephen J Redman
Mar 30, 2000·Progress in Neurobiology·M R Bennett, J L Kearns
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Céline BidoretMariano Casado
Oct 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·M CasadoP Ascher
Sep 14, 2000·Proceedings of the National Academy of Sciences of the United States of America·N Schweighofer, G Ferriol
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Nov 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Wataru Kakegawa, Michisuke Yuzaki
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