Assessment of the role of activin A and transforming growth factor beta in the regulation of AML12 cell growth

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Y Q ZhangI Kojima

Abstract

The present study was conducted to determine the role of two autocrine factors, activin A and transforming growth factor beta (TGF-beta), in the growth regulation of AML12 hepatocytes. We overexpressed truncated type II activin and/or TGF-beta receptors in AML12 cells. In AML12 cells overexpressing truncated type II activin receptors (AML-tAR cells), the inhibitory effect of activin A on DNA synthesis was completely blocked. AML-tAR cells proliferated faster than parental cells, both in the presence and absence of epidermal growth factor (EGF). However, AML-tAR cells could not grow in soft agar. Follistatin augmented EGF-induced DNA synthesis in AML12 cells, whereas it was ineffective in AML-tAR cells. In AML12 cells overexpressing truncated type II TGF-beta receptor (AML-tTR cells), the inhibitory effect of TGF-beta on DNA synthesis was blocked. AML-tTR cells proliferated faster than parental cells, both in the presence and absence of EGF, but at a slower rate than that of AML-tAR cells. AML-tTR cells did not grow in soft agar. The growth rate of cells overexpressing both types of truncated receptors was identical to that of AML-tAR cells, and these cells did not grow in soft agar. These results indicate that both activin A an...Continue Reading

Citations

Feb 19, 2000·Biochemical and Biophysical Research Communications·A MaeshimaI Kojima
Jun 1, 2005·Cytokine & Growth Factor Reviews·Christopher M ButlerGail P Risbridger
Jul 14, 2001·Molecular and Cellular Endocrinology·I KojimaY Q Zhang
Mar 25, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K KogureI Kojima
Jan 31, 2002·World Journal of Gastroenterology : WJG·X HuangH M Lu
Mar 25, 2004·American Journal of Physiology. Endocrinology and Metabolism·Wataru WadaItaru Kojima
Jan 30, 2002·Experimental Biology and Medicine·Ye-Guang ChenShao-Yao Ying

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