Assessment of the safety and efficacy of an attenuated live vaccine based on highly pathogenic porcine reproductive and respiratory syndrome virus

Clinical and Vaccine Immunology : CVI
Xiuling YuKegong Tian

Abstract

The safety and efficacy of the JXA1-R vaccine, an attenuated strain of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV), were examined using an intramuscular challenge model in piglets. The JXA1-R vaccine was obtained by passing HP-PRRSV JXA1 through Marc-145 cells (82nd passage). Genomic sequence comparisons showed that strain JXA1-R and its parental strain, JXA1, differ by 47 amino acids, and most of these differences are scattered throughout the PRRSV genome. Four-week-old PRRSV-free piglets were inoculated intramuscularly with JXA1-R vaccine (10(3.0), 10(4.0), 10(5.0), 10(6.0), and 10(7.0) 50% tissue culture infective doses [TCID50]/ml for groups 1 to 5, respectively) and then challenged intramuscularly with the 5th passage virus of JXA1 virus (JXA1-F5, 3 ml × 10(4.5) TCID50/ml) 28 days after inoculation. The humoral immune response, swine growth, clinical signs, and differential organ lesions were monitored. The results showed that all vaccinated piglets had a perceptible humoral immune response to vaccination after day 7, which then promptly increased, almost reaching the maximum sample/positive (S/P) ratio value at 28 days postimmunization. Viremia detection indicated that the viral replic...Continue Reading

References

May 29, 1998·The Journal of General Virology·E J Snijder, J J Meulenberg
Feb 9, 2000·Archives of Virology·T StorgaardA Bøtner
Feb 17, 2000·The Journal of Veterinary Medical Science·I ShibataS Yazawa
Feb 7, 2008·Emerging Infectious Diseases·Guang-Zhi TongHua-Ji Qiu
Nov 4, 2008·Emerging Infectious Diseases·Youjun FengGeorge F Gao
Feb 11, 2011·The Journal of General Virology·Andrew E FirthClara C Posthuma
Mar 2, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Jianqiang NiKegong Tian

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Citations

Apr 27, 2018·Tropical Animal Health and Production·Chaitawat SirisereewanKomkrich Teankum

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Software Mentioned

Clustal X
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