Assessment of the sensitization potential of five metal salts in the murine local lymph node assay

Toxicology
C ManderveltB Nemery

Abstract

The murine local lymph node assay (LLNA) has been proposed as a predictive test for the identification of sensitizing agents. We used this test to compare the sensitization potential of NiSO4, K2Cr2O7, CoCl2, Na2PtCl6 and BeSO4, salts of metals which have all been associated with allergic contact dermatitis and either bronchial asthma orinterstitial lung disease, by either humoral or cell-mediated allergic mechanisms. BALB/c mice (n = 3 per concentration studied, three concentrations studied per metal) received three daily applications of the metal salt (in DMSO) on the dorsum of both ears. On the fourth day the draining auricular lymph nodes were removed and the incorporation of [3H]-thymidine in the lymphocytes in culture was compared to that of concurrent vehicle-treated control mice, thus enabling to derive a stimulation index (SI), indicative of immunological sensitization potential. Each experiment was performed three times. Oxazolone and toluene diisocyanate, chosen as positive controls, yielded strongly positive SI values (> 20 and > 30 respectively). Na2PtCl6 (SI 2.6 +/- 1.0 at 2.5%), CoCl2 (SI 2.8 +/- 0.5 at 5%) and possibly also K2Cr2O7 (SI 2.1 +/- 1.2 at 0.5%) were positive in the LLNA, whereas NiSO4 (SI 0.9 +/- 0.2...Continue Reading

References

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Citations

Nov 7, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Joanna ArkuszElzbieta Dziubałtowska
Apr 15, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Hendrik ReuterAndreas Schepky
May 16, 2015·Archives of Toxicology·Thomas Armin FrohweinMaximilian Schreiner
Nov 15, 2018·Contact Dermatitis·Trine H PetersenCharlotte M Bonefeld
Sep 12, 2019·Journal of Toxicology and Environmental Health. Part a·Mahesh Raj NepalTae Cheon Jeong

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