Abstract
Most food allergy cases are associated with a limited group of allergens. This could be attributed to an increased ability of some foods to sensitize and trigger allergic reactions. However, there are no validated animal models to evaluate the sensitizing or allergenic potentials of proteins. Our aim was to evaluate three protocols of adjuvant-free intraperitoneal sensitization that differ in the time points for sample collection (days 14, 28 and 35 from beginning of the sensitization) and also in the number of immunizations (2, 5 and 3, respectively). Ovalbumin (OVA; 0.05 mg), cow milk proteins (CMP; 0.025, 0.05 and 0.25 mg), and potato acid phosphatase (PAP; low allergenic protein; 250.0 mg) were administered intraperitoneally (ip) to BALB/c mice (n = 4⁻6) and the protein-specific IgE and IgG antibody responses were evaluated using ELISA. Additional serum protein-specific IgE antibodies evaluations were carried out after IgG depletion. Anti-OVA IgE antibodies were detected in mice from all three protocols. The responses were higher in the group of mice that underwent the 28-day protocol than in those that underwent the 14- or 35-day protocols (p < 0.01 and p < 0.05, respectively). Anti-CMP IgE antibodies were detected in both...Continue Reading
References
Aug 15, 1970·Nature·U K Laemmli
Jul 1, 1994·Toxicology Letters·J HiltonI Kimber
Feb 4, 1998·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J HiltonI Kimber
Apr 27, 2001·Toxicology Letters·R J Dearman, I Kimber
Oct 2, 2001·Toxicology·R J DearmanI Kimber
Jun 21, 2002·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ian Kimber, Rebecca J Dearman
Apr 2, 2003·Journal of Immunological Methods·Neil BirminghamVenu Gangur
May 16, 2003·Toxicology·Patrick J Haley
Apr 9, 2005·Allergy·K Adel-PatientJ-M Wal
Dec 13, 2006·Methods : a Companion to Methods in Enzymology·Rebecca J Dearman, Ian Kimber
Jul 14, 2007·The Journal of Allergy and Clinical Immunology·Roberto J RonaCharlotte Madsen
Feb 28, 2008·Nature Reviews. Immunology·Hannah J Gould, Brian J Sutton
Dec 23, 2008·Regulatory Toxicology and Pharmacology : RTP·Hatice AldemirCorinne Herouet-Guicheney
Sep 19, 2009·Journal of Dairy Science·B GonipetaV Gangur
Oct 6, 2009·Regulatory Toxicology and Pharmacology : RTP·G S LadicsC Herouet-Guicheney
May 13, 2010·JAMA : the Journal of the American Medical Association·Jennifer J Schneider ChafenPaul G Shekelle
Jul 10, 2010·Lung India : Official Organ of Indian Chest Society·Sudha S DeoPramod V Niphadkar
Nov 3, 2011·Molecular Nutrition & Food Research·Stéphanie MorinStéphane Hazebrouck
Jun 12, 2013·Journal of Applied Toxicology : JAT·Rebecca J DearmanIan Kimber
Aug 24, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Chen ChenXudong Jia
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·Babu GonipetaVenu Gangur
Jan 14, 2016·Molecular Immunology·Tiange LiuAndreas L Lopata
Dec 21, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·B RemingtonA Constable
Citations
Mar 23, 2019·Medicina·Feliznando Isidro Cárdenas-TorresFrancisco Cabrera-Chávez
Aug 9, 2019·Food & Function·Meng LiuGuang-Ming Liu
Sep 7, 2019·Nutrients·Ewa FucBarbara Wróblewska
Feb 23, 2020·Journal of Food Science·Ping TongHongbing Chen