Assessment of tissue-engineered stomach derived from isolated epithelium organoid units

Transplantation Proceedings
Tomoyuki MaemuraJ P Vacanti

Abstract

Isolated stomach epithelial organoid units developed on biodegradable polymers were transplanted to assess the feasibility of a tissue-engineered stomach. Despite recent advances in reconstruction techniques, total gastrectomy is still accompanied by various complications. An alternative treatment would be a tissue-engineered stomach, which replaces the mechanical and metabolic functions of a normal stomach. Stomach epithelial organoid units isolated from neonatal rats were seeded onto biodegradable polymers. The constructs implanted into the omenta of adult rats were harvested for examination at designated times. Nine rats underwent a second operation for anastomosis. The constructs resulted in cyst-like formations showing vascularized tissue with neomucosa lining the lumen. The surface morphology as assessed using scanning electron microscopy was similar to that of a native stomach. Immunohistochemical staining for alpha-actin smooth muscle and gastric mucin indicated the presence of a smooth muscle layer and a well-developed gastric epithelium, respectively. The luminal surface of the anastomosed tissue-engineered stomach was well-covered with epithelium. Epithelium-derived stomach organoid units seeded on biodegradable poly...Continue Reading

References

Jan 21, 1991·International Journal of Cancer. Journal International Du Cancer·J BaraN Daher
Mar 20, 1999·Transplantation Proceedings·S S KimJ P Vacanti
Mar 20, 1999·Transplantation Proceedings·S KaiharaJ P Vacanti
Oct 21, 1999·The Journal of Surgical Research·S S KimJ P Vacanti

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Citations

Sep 12, 2006·Biomedizinische Technik. Biomedical Engineering·Dorothee RickertRalf-Peter Franke
Jul 11, 2012·World Journal of Surgery·Victor W WongMichael T Longaker
May 17, 2011·The Journal of Surgical Research·Allison L SpeerTracy C Grikscheit
Mar 15, 2006·Dental Clinics of North America·Pamela C Yelick, Joseph P Vacanti
Nov 2, 2011·Artificial Organs·Tomoyuki MaemuraDaizoh Saitoh
Apr 30, 2013·The Journal of Surgical Research·Tomoyuki MaemuraManabu Kinoshita
Oct 23, 2004·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Tomoyuki MaemuraJoseph P Vacanti
Dec 26, 2006·Expert Review of Medical Devices·Rebecca A Penkala, Stephen S Kim
Mar 19, 2019·Expert Opinion on Biological Therapy·Elie ZakhemKhalil N Bitar
Aug 8, 2012·Science and Technology of Advanced Materials·Sumit PramanikNoor Azuan Abu Osman

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