Assessment of Toxoplasma gondii levels in zebra mussel (Dreissena polymorpha) by real-time PCR: an organotropism study

Environmental Science and Pollution Research International
M Palos LadeiroA Geffard

Abstract

Water quality is a public health concern that calls for relevant biomonitoring programs. Molecular tools such as polymerase chain reaction (PCR) are progressively becoming more sensitive and more specific than conventional techniques to detect pathogens in environmental samples such as water and organisms. The zebra mussel (Dreissena polymorpha) has already been demonstrated to accumulate and concentrate various human waterborne pathogens. In this study, first, a spiking experiment to evaluate detection levels of Toxoplasma gondii DNA in zebra mussel organs using real-time PCR was conducted. Overall, lower DNA levels in the hemolymph, digestive gland, and remaining tissues (gonad and foot) were detected compared to mantle, muscle, and gills. Second, an in vivo experiment with 1000 T. gondii oocysts per mussel and per day for 21 consecutive days, followed by 14 days of depuration time in protozoa-free water was performed. T. gondii DNA was detected in all organs, but greatest concentrations were observed in hemolymph and mantle tissues compared to the others organs at the end of the depuration period. These results suggest that (i) the zebra mussel is a potential new tool for measuring T. gondii concentrations and (ii) real-time...Continue Reading

References

Dec 13, 2000·International Journal for Parasitology·A M TenterL M Weiss
Mar 22, 2002·The Journal of Eukaryotic Microbiology·D S LindsayJ P Dubey
Jul 9, 2002·Environmental Science & Technology·Frank J LogeDouglas R Call
Sep 18, 2003·International Journal for Parasitology·Kristen D ArkushPatricia A Conrad
Jun 3, 2004·The Lancet Infectious Diseases·Samuel Yang, Richard E Rothman
Jul 9, 2004·Applied and Environmental Microbiology·Isabelle VillenaJean-Michel Pinon
Nov 30, 2004·Veterinary Parasitology·J P Dubey
Dec 9, 2008·Applied and Environmental Microbiology·Karen ShapiroPatricia A Conrad
Mar 28, 2009·Experimental Parasitology·J L Jones, J P Dubey
Aug 14, 2010·Water Science and Technology : a Journal of the International Association on Water Pollution Research·P RoslevN Iversen
Oct 21, 2011·Nucleic Acids Research·Tzachi BarAles Tichopad
Dec 14, 2011·Applied and Environmental Microbiology·Aurélien DumètreIsabelle Villena
May 9, 2012·Water Research·Hyatt C Green, Katharine G Field
Sep 25, 2012·Analytical and Bioanalytical Chemistry·Marelize BotesThomas Eugene Cloete
Sep 25, 2012·Environmental Science and Pollution Research International·M Palos LadeiroA Geffard
Dec 18, 2013·Veterinary Parasitology·Meng ZhangXianRrui Li

❮ Previous
Next ❯

Citations

Jul 15, 2015·Environmental Science and Pollution Research International·Denis FaureRobert Duran
Aug 28, 2015·Environmental Science and Pollution Research International·Denis FaureUNKNOWN Microbial Ecology EC2CO consortium
Sep 1, 2016·Folia Parasitologica·John Asekhaen Ohiolei, Clement Isaac
Nov 20, 2018·Environmental Microbiology Reports·Antoine Le GuernicMélissa Palos Ladeiro
Jan 5, 2019·Parasitology Research·Ana Tereza GalvaniMaria Tereza Pepe Razzolini
Oct 29, 2020·Journal of Environmental Management·Elodie GébaAurélie Bigot-Clivot
Jun 22, 2021·Journal of Applied Microbiology·Aurélie Bigot-ClivotAlain Geffard

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
O KupferschmidtK Janitschke
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
Z H GengJ H Li
© 2021 Meta ULC. All rights reserved