Assignment of the 31P and 1H resonances in oligonucleotides by two-dimensional NMR spectroscopy

FEBS Letters
V SklenárA Bax

Abstract

The use of new 1H-detected heteronuclear 1H-31P shift correlation experiments is demonstrated for oligonucleotides of 12 and 40 base pairs. The methods give unambiguous assignments of the 31P resonances and also permit identification of the C4' and C5' sugar protons. Use of the new methods enables one to make sequence-specific resonance assignments without reference to a known or assumed conformation of the DNA fragment.

Citations

Oct 1, 1989·International Journal of Biological Macromolecules·V SklenárM Vorlícková
Feb 1, 1991·International Journal of Biological Macromolecules·J KyprJ Smrt
Dec 1, 1988·Journal of Biochemical and Biophysical Methods·G Remaud
Dec 4, 2003·Journal of Molecular Biology·John W StuartPaul F Agris
May 24, 2005·Journal of Molecular Biology·Cyril GaudinDominique Fourmy
Nov 8, 2002·Journal of Molecular Biology·Dominique FourmySatoko Yoshizawa
Jan 16, 2003·Journal of Molecular Biology·Pramodh Vallurupalli, Peter B Moore
May 22, 2003·Journal of Molecular Biology·Ko-Hsin Chin, Shan-Ho Chou
Apr 20, 2001·International Journal of Biological Macromolecules·E HantzT Huynh Dinh
Nov 11, 1991·Quarterly Reviews of Biophysics·G Varani, I Tinoco
Aug 1, 1987·Quarterly Reviews of Biophysics·D J PatelD Hare
Mar 30, 2011·Biochemistry·Christopher N JohnsonMarkus W Germann
Feb 25, 2009·Journal of the American Chemical Society·Yongjun ChuBrent L Iverson
Apr 18, 1998·Nature Structural Biology·M BachelinH Kessler
Oct 23, 1997·Nature Structural Biology·E V PuglisiJ D Puglisi
Sep 22, 1999·European Journal of Biochemistry·R P OjhaR H Sarma
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Shan-Ho ChouFu-Ming Chen
Sep 13, 2001·Proceedings of the National Academy of Sciences of the United States of America·S H BaeB S Choi
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·F W SmithJ Feigon
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·L ZhuB R Reid
Feb 1, 1988·Journal of Biomolecular Structure & Dynamics·M DelepierreJ Igolen
Jan 24, 1998·Journal of Biomolecular Structure & Dynamics·N JingM E Hogan
Aug 19, 2000·Journal of Biomolecular Structure & Dynamics·S SmirnovC de los Santos
Jan 1, 2000·Journal of Biomolecular Structure & Dynamics·P AichJ S Lee
Sep 22, 2001·Journal of Biomolecular Structure & Dynamics·U DornbergerH Fritzsche
Jan 16, 2002·Journal of Biomolecular Structure & Dynamics·C El AmriS Fermandjian
Jun 25, 2004·Journal of Biomolecular Structure & Dynamics·X MaoW H Gmeiner
Feb 21, 1998·The EMBO Journal·S E ButcherJ Feigon
Jun 25, 1995·Nucleic Acids Research·R J CainG D Glick
Dec 11, 1995·Nucleic Acids Research·S AltmannW Leupin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.