Assimilation of nitrogen and carbon isotopes from fish diets to otoliths as measured by nanoscale secondary ion mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Jen-Chieh ShiaoYung-Che Tseng

Abstract

Nitrogen and carbon stable isotope ratios (δ15 N and δ13 C values) of carbonate-bound organic materials in otoliths can provide information to address the biological and ecological functions of fish. Correct interpretation of otolith δ15 N and δ13 C profiles requires knowledge of the metabolic routes of nitrogen and carbon isotopes. However, the isotopic assimilation of δ15 N and δ13 C compositions from diets to otoliths has rarely been investigated. This study traced the daily nitrogen and carbon isotopic assimilation between diets and otoliths using nanoscale secondary ion mass spectrometry (NanoSIMS). Isotopically labeled algae (Tetraselmis chui) were fed to tilapia (Oreochromis niloticus) for 14-17 days. NanoSIMS and conventional isotope ratio mass spectrometry were used to measure δ15 N and δ13 C variations in the otoliths and fish muscle, respectively. Otolith δ15 N values abruptly surged from natural abundance levels by 1000-2300‰ after the fish ate 15 N-spiked algae with δ15 N values of approximately 2200‰. However, the δ15 N values of fish muscle increased to only approximately 500‰ at the end of the feeding experiment. Much higher δ15 N values (3700-14 000‰) and moderate δ13 C values (60-200‰) were detected in the oto...Continue Reading

References

Oct 1, 1973·Life Sciences·C T GreggJ E Furchner
Aug 1, 1997·European Journal of Pediatrics·B KoletzkoH Demmelmair
Mar 30, 2004·Journal of the American Society for Mass Spectrometry·R Peteranderl, C Lechene

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