Assimilation of oxalate, acetate, and CO2 by Oxalobacter formigenes

Canadian Journal of Microbiology
N A Cornick, M J Allison

Abstract

Oxalobacter formigenes is the only well-documented oxalate-degrading bacterium isolated from the gastrointestinal tract of animals. The production of ATP by Oxalobacter formigenes is centered around oxalate metabolism and oxalate is required for growth. A small amount of acetate (0.5 mM) is also required. Oxalate is decarboxylated to formate plus CO2 in nearly equimolar amounts. Experiments were conducted to determine which potential carbon sources (oxalate, acetate, formate, CO2) were assimilated by Oxalobacter formigenes and which metabolic pathways were operative in carbon assimilation. Measurements of the specific activities of total cell carbon after growth with different 14C-labeled precursors indicated that at least 54% of the total cell carbon was derived from oxalate and at least 7% was derived from acetate. Carbonate was also assimilated, but formate was not a significant source of cell carbon. Labeling patterns in amino acids from cells grown in [14C]oxalate or 14CO3 were different; however, in both cases 14C was widely distributed into most cellular amino acids. Carbon from [14C]acetate was less widely distributed and detected mainly in those amino acids known to be derived from alpha-ketoglutarate, oxaloacetate, an...Continue Reading

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Citations

Jan 22, 2004·FEMS Microbiology Letters·Colin S StewartDavid R Cave
Aug 2, 2003·Research in Microbiology·Nurettin Sahin
Nov 27, 2008·Progrès en urologie : journal de l'Association française d'urologie et de la Société française d'urologie·F BruyereE Lechevallier
Jun 24, 2004·The Journal of Biological Chemistry·Stefan JonssonNigel G J Richards
Jul 14, 2012·Journal of Applied Microbiology·J S GnanandarajahM P Murtaugh
May 31, 2019·Current Opinion in Nephrology and Hypertension·Menghan Liu, Lama Nazzal
May 13, 2010·FEMS Microbiology Ecology·Kristine Michelle L Hutalle-SchmelzerHans-Peter Grossart
Jun 20, 2020·Bulletin of Environmental Contamination and Toxicology·Yuanyuan YanCheng Gu
Jan 27, 2019·Scientific Reports·Amanda PeBenitoMartin J Blaser
Dec 31, 2020·Indian Journal of Urology : IJU : Journal of the Urological Society of India·Ajay P SharmaSumit Dave
Mar 15, 2001·Biochimica Et Biophysica Acta·W Buckel
Dec 8, 2004·Archives of Biochemistry and Biophysics·Drazenka SvedruzićNigel G J Richards
Jul 1, 2021·Applied and Environmental Microbiology·Steven L DanielJohn Knight

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