Associated Detection of Superoxide Anion and Mercury(II) under Chronic Mercury Exposure in Cells and Mice Models via a Three-Channel Fluorescent Probe

Analytical Chemistry
Yue WangLingxin Chen

Abstract

As a cytotoxic heavy metal ion, mercury(II) ion (Hg2+) induces severe oxidative stress and further results in physiological dysfunction. Although mercury poisoning can be treated with many drugs, such as sodium selenite, the therapeutic effect is relatively poor, and it seems that the damage to human health continues. However, the interpretation for the pathogenesis has not been clarified yet. We supposed that the reason is attributed to Hg2+-caused intracellular oxidative stress. To confirm our hypothesis, we strived to design a three-channel ratio fluorescent probe, HCy-SeH, for superoxide anion (O2•-) and Hg2+ combined detection. O2•- is a vital precursor for other reactive oxygen species (ROS), which is involved in many physiological and pathological processes. However, until now there is no efficient chemical tool for O2•- and Hg2+ combined detection in cells and in vivo. The fluorescence response of our probe is initiated by a hydrogen abstraction reaction from the hydrocyanine fluorophore moiety. Once oxidized by O2•-, HCy-SeH recovers its π-conjugated system back to a heptamethine cyanine derivative, Cy-SeH. Cy-SeH coexists with its conjugate base, Cy═Se. One emits red fluorescence, and the other one emits green fluores...Continue Reading

References

Nov 1, 1983·Bulletin of Environmental Contamination and Toxicology·Y M SinM K Wong
Mar 4, 2000·Toxicological Sciences : an Official Journal of the Society of Toxicology·P L GoeringR J Marler
Nov 14, 2000·Circulation Research·H Cai, D G Harrison
Aug 6, 2004·Nature·Mark P Mattson
Apr 12, 2005·Annual Review of Pharmacology and Toxicology·John D HayesIan R Jowsey
Oct 26, 2006·Journal of the American Chemical Society·Sung-Kyun KoInjae Shin
Apr 19, 2008·Nature Chemical Biology·Christine C Winterbourn
Sep 17, 2008·Angewandte Chemie·Xiaolin ZhangXuhong Qian
Dec 4, 2008·Journal of the American Chemical Society·Fengling SongKazunori Koide
Jun 3, 2009·The Journal of Clinical Investigation·Raghu Kalluri, Robert A Weinberg
Feb 8, 2011·Journal of the American Chemical Society·Shin Ando, Kazunori Koide
Feb 11, 2011·Organic Letters·Issa SambIsabelle Leray
Mar 2, 2011·Organic & Biomolecular Chemistry·Yuanyuan LiXianshun Zeng
Jun 2, 2011·Chemical Society Reviews·Xiaoqiang ChenJuyoung Yoon
Jul 20, 2011·Nature Chemical Biology·Bryan C Dickinson, Christopher J Chang
Jul 30, 2011·Nature Methods·Tasuku Ueno, Tetsuo Nagano
Dec 21, 2011·Chemical Society Reviews·Ha Na KimJuyoung Yoon
Jul 7, 2012·Chemical Communications : Chem Comm·Jiangli FanXiaojun Peng
Sep 18, 2012·Journal of the American Chemical Society·Quanjuan ZhangYoujun Yang
Sep 26, 2013·Journal of the American Chemical Society·Wen ZhangBo Tang
Oct 7, 2014·Chemical Society Reviews·Min Hee LeeJonathan L Sessler
Feb 14, 2015·Chemical Communications : Chem Comm·Chao SongWei Wang
May 20, 2015·Journal of the American Chemical Society·Jun Jacob HuDan Yang

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Citations

Nov 22, 2019·Chemical Society Reviews·Sang-Hyun ParkInjae Shin
Jan 15, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yue WangLingxin Chen
Jun 12, 2020·Chemical Communications : Chem Comm·Huawei NiuYufen Zhao
Oct 23, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Sansan ShenHong Wang
Dec 11, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Deblina SarkarPrasanta Kumar Das
Dec 29, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Lu SunLi Xu

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