Association and in silico assignment of sequences from turkey BACs

Animal Biotechnology
K M ReedLaura M Sullivan

Abstract

Bacterial artificial chromosomes (BACs) provide an important resource in genetic mapping. An initial set of BACs corresponding to microsatellite markers in the turkey (Meleagris gallopavo) was isolated from the CHORI-260 turkey BAC library. The selected markers were distributed on both macro- and microchromosomes and included a genetically unlinked marker. End sequences were obtained for a subset of the recovered BACs and compared to the chicken whole genome sequence. Close association of the turkey BAC-end sequences and original marker sequences was generally conserved in the chicken genome. Gene content of the turkey BACs is predicted from the comparative sequence alignments.

References

May 3, 2000·Mammalian Genome : Official Journal of the International Mammalian Genome Society·R P CrooijmansM A Groenen
Jan 2, 2004·Animal Biotechnology·K M ReedA J Torgerson
Jan 13, 2005·Genome Génome / Conseil National De Recherches Canada·Todd P KnutsonKent M Reed
Oct 27, 2006·Cytogenetic and Genome Research·K M ReedF A Ponce de León
Dec 28, 2007·Cytogenetic and Genome Research·K M ReedK M Mendoza

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Methods Mentioned

BETA
PCR

Software Mentioned

Ensembl
BLAST

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