Association between actin and light chains in Chlamydomonas flagellar inner-arm dyneins

Biochemical and Biophysical Research Communications
H A Yanagisawa, R Kamiya

Abstract

Inner dynein arms in cilia and flagella contain actin as a subunit; however, the function of this actin is totally unknown. Here we performed chemical crosslinking experiments to examine the interaction of actin with other subunits. Six of the seven Chlamydomonas inner-arm dynein species separated by anion-exchange chromatography contain actin and either one of the two previously identified light chains, p28 and centrin, in a mutually exclusive manner. Western blotting of chemically crosslinked dyneins indicated that actin is directly associated with p28 and centrin but not with the dynein heavy chains (HCs). In contrast, p28 and centrin both appeared to interact directly with the N-terminal half of the HCs. Thus it is likely that actin is associated with the heavy chains through p28/centrin. These light chains may well function in the assembly or targeting of the inner arm to the correct axonemal location.

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Citations

Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Alexander R ParedezW Z Cande
Nov 6, 2007·Eukaryotic Cell·Ryosuke YamamotoRitsu Kamiya
Mar 24, 2004·Journal of Structural Biology·Miho Sakato, Stephen M King
Mar 24, 2004·Journal of Structural Biology·S A BurgessP J Knight
Jul 7, 2010·The Journal of Cell Biology·Ryosuke YamamotoRitsu Kamiya
Nov 26, 2008·The Journal of Cell Biology·Khanh Huy BuiTakashi Ishikawa
Nov 11, 2006·FEBS Letters·Ryosuke YamamotoRitsu Kamiya
Sep 19, 2003·Biomedical Chromatography : BMC·Tibor CserhátiAdam Echardt
Sep 17, 2008·Molecular Microbiology·T V Satish TammanaChhitar M Gupta
Jul 23, 2011·The FEBS Journal·Hitoshi Sakakibara, Kazuhiro Oiwa
May 24, 2014·Biophysical Journal·Youské ShimizuKazuhiro Oiwa
Dec 3, 2003·Cell Motility and the Cytoskeleton·Elizabeth F Smith, Pinfen Yang
Apr 15, 2014·Eukaryotic Cell·Alexander R ParedezW Zacheus Cande
Jun 4, 2010·Nature Structural & Molecular Biology·Stephen M King
Feb 24, 2006·Molecular Reproduction and Development·Sajid RashidJuergen Neesen
Aug 3, 2017·Cold Spring Harbor Perspectives in Biology·Rasagnya ViswanadhaMary E Porter
Aug 10, 2002·Molecular & Cellular Proteomics : MCP·Lawrence E OstrowskiRichard C Boucher
Apr 25, 2020·International Journal of Molecular Sciences·Kenta IshibashiKazuhiro Oiwa
Dec 16, 2020·Nature Structural & Molecular Biology·Miao GuiAlan Brown
Jan 22, 2021·Nature Communications·Travis WaltonAlan Brown
May 27, 2010·Journal of Cell Science·Santharam S KattaChhitar M Gupta
Jul 22, 2021·Microbiology Spectrum·Melissa C Steele-OgusAlexander R Paredez

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