Association between avian necrotic enteritis and Clostridium perfringens strains expressing NetB toxin.

Veterinary Research
Anthony L KeyburnRobert J Moore

Abstract

A novel toxin, NetB, has recently been identified in virulent avian Clostridium perfringens isolates and shown to be an essential virulence factor in a clinical necrotic enteritis isolate. To assess whether NetB is more generally associated with avian necrotic enteritis isolates we have screened a range of C. perfringens strains from geographically diverse locations for both the presence and expression of the netB gene. Forty-four isolates were derived from necrotic enteritis disease cases from Australia, Belgium, Denmark and Canada and 55 isolates from healthy chickens from Australia and Belgium. The majority of strains isolated from necrotic enteritis-affected birds were netB positive (70%) and there was an absolute correlation between the presence of netB and in vitro expression of the NetB protein. Only two of the C. perfringens isolates from healthy chickens carried netB. Sequencing of the netB gene from 23 positive isolates showed that NetB is highly conserved, with only one predicted amino acid (A168T) difference, in six isolates, compared to the published sequence. This change did not alter the in vitro activity of the NetB toxin. The gene encoding the recently discovered TpeL toxin was also screened using PCR and only ...Continue Reading

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Citations

Aug 5, 2011·Avian Pathology : Journal of the W.V.P.A·L TimbermontF Van Immerseel
Jul 20, 2013·Veterinary Research·Anthony L KeyburnRobert J Moore
Apr 26, 2014·Future Microbiology·Francisco A UzalBruce A McClane
Jan 27, 2015·Avian Diseases·Marta Brito GuimarãesTerezinha Knöbl
Jul 2, 2014·Avian Pathology : Journal of the W.V.P.A·Dorien MotFilip Van Immerseel
May 31, 2016·Scientifica·Sajid UmarMuhammad Tanveer Munir
Mar 25, 2016·Avian Pathology : Journal of the W.V.P.A·Julian I RoodRobert J Moore
Mar 2, 2016·Avian Pathology : Journal of the W.V.P.A·Robert J Moore
Mar 8, 2016·Avian Pathology : Journal of the W.V.P.A·Jake A LaceyRobert J Moore
Oct 7, 2014·Research in Microbiology·John C FreedmanBruce A McClane
Apr 11, 2013·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Kerry K CooperFrancisco A Uzal
May 23, 2018·Toxins·Mauricio A NavarroFrancisco A Uzal
May 24, 2013·Microbiology and Molecular Biology Reviews : MMBR·Jihong LiBruce A McClane
Jan 12, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Ben WadeRobert J Moore
May 23, 2020·Avian Pathology : Journal of the W.V.P.A·Evy GoossensFilip Van Immerseel
Oct 28, 2019·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Francesca ProfetaFulvio Marsilio

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Methods Mentioned

BETA
PCR

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