Association between Biofilm-Production and Antibiotic Resistance in Uropathogenic Escherichia coli (UPEC): An In Vitro Study

Diseases
Payam BehzadiMárió Gajdács

Abstract

Urinary tract infections (UTIs) are among the most common infections requiring medical attention worldwide. The production of biofilms is an important step in UTIs, not only from a mechanistic point of view, but this may also confer additional resistance, distinct from other aspects of multidrug resistance (MDR). A total of two hundred and fifty (n = 250) Escherichia coli isolates, originating from clean-catch urine samples, were included in this study. The isolates were classified into five groups: wild-type, ciprofloxacin-resistant, fosfomycin-resistant, trimethoprim-sulfamethoxazole-resistant and extended spectrum β-lactamase (ESBL)-producing strains. The bacterial specimens were cultured using eosine methylene blue agar and the colony morphology of isolates were recorded. Antimicrobial susceptibility testing was performed using the Kirby-Bauer disk diffusion method and E-tests. Biofilm-formation of the isolates was carried out with the crystal violet tube-adherence method. n = 76 isolates (30.4%) produced large colonies (>3 mm), mucoid variant colonies were produced in n = 135 cases (54.0%), and n = 119 (47.6%) were positive for biofilm formation. The agreement (i.e., predictive value) of mucoid variant colonies in regard t...Continue Reading

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Citations

Jul 16, 2020·Antibiotics·Meysam SarsharDaniela Scribano
Nov 12, 2020·International Journal of Environmental Research and Public Health·Fariani SyahrulFebi Dwirahmadi
Oct 30, 2020·Infection and Drug Resistance·Wenli LiaoTieli Zhou
Jul 14, 2021·Applied Microbiology and Biotechnology·Edgar González-VillalobosJosé Molina-López
Aug 26, 2021·Infection and Drug Resistance·Rasha El-MahdyRaghdaa Shrief

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Methods Mentioned

BETA
pharmacotherapy
electron microscopy

Software Mentioned

SPSS Statistics
MALDI Biotyper RTC
Excel

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