Association between cigarette smoking and urinary excretion of 1,N2-ethenoguanine measured by isotope dilution liquid chromatography-electrospray ionization/tandem mass spectrometry

Chemical Research in Toxicology
Hauh-Jyun Candy Chen, Wei-Loong Chiu

Abstract

Levels of the promutagenic 1,N2-ethenoguanine (1,N2-epsilonGua), an etheno DNA adduct derived mainly from lipid peroxidation, in experimental animals are associated with risk of cancer formation. Since 1,N2-epsilonGua can be repaired by human glycosylases, it is possible to use it as a biomarker for cancer risk assessment in humans. In the present study, a highly sensitive and specific stable isotope dilution liquid chromatography-electrospray ionization/tandem mass spectrometry (LC-ESI/MS/MS) was developed for accurate quantification of 1,N2-epsilonGua in human urine. The sample pretreatment involved a consecutive strong cation exchange solid-phase extraction (SPE) and reversed phase SPE chromatography. The pretreated sample was analyzed by LC-ESI/MS/MS under multiple reaction monitoring mode (MRM) using a triple quadrupole mass spectrometer. The detection limit of 1,N2-epsilonGua using this LC-ESI/MS/MS assay was 1.0 pg (5.8 fmol) injected on-column. Levels of urine samples collected from healthy volunteers were found to range from 0 to 199 pg/mL, and levels as low as 5.0 pg/mL (29 pM) could be accurately quantified. After adjusting for creatinine levels and body weight, an statistically significant association was observed b...Continue Reading

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Citations

Mar 1, 2006·Journal of the American Society for Mass Spectrometry·Peter R HillestrømHenrik E Poulsen
Jul 26, 2012·Chemical Research in Toxicology·Natalia TretyakovaGregory Janis
Jan 15, 2011·Journal of Nucleic Acids·Bo Hang
Feb 28, 2013·Chemical Reviews·Natalia TretyakovaSrikanth Kotapati
Feb 23, 2012·Free Radical Research·Allan WeimannHenrik Enghusen Poulsen
Nov 21, 2007·Journal of Molecular Biology·Alexandra DimitriDavid A Scicchitano
Jul 29, 2011·Molecular Nutrition & Food Research·Hauh-Jyun Candy Chen
Feb 14, 2021·Redox Biology·Mu-Rong ChaoMarcus S Cooke
Jan 10, 2009·Biochemistry·Charles G KnutsonLawrence J Marnett
Jun 17, 2021·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·F Peter Guengerich, Pratibha P Ghodke
Feb 22, 2007·Chemical Research in Toxicology·Charles G KnutsonLawrence J Marnett

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